Huskić J, Kulenović H
Institut za fiziologiju i biohemiju, Medicinski fakultet, Sarajevo.
Med Arh. 1995;49(3-4):75-8.
The effects of duration of disease on serum angiotensin converting enzyme (ACE) was measured in 60 patients with endemic nephropathy (30 men and 30 women) of age between 30 and 60 years. There were formed three groups: patients with endemic nephropathy and duration of disease less than 5 years (n = 23), patients with endemic nephropathy and duration of disease between 5-10 years (n = 17); and patients with endemic nephropathy and duration of disease 10 years and more (n = 20). The serum ACE activity was determined by the spectrophotometric method using Hip-Gly-Gly as a substrate. The activity of enzyme were expressed in units corresponding to 1 nmol of the hippuric acid that was released by the hydrolysis of Hip-Gly-Gly per minute and ml of serum. The results showed that serum ACE activity decreased in group of patients with endemic nephropathy and duration of disease 10 years and more (29.21 +/- 2.25; X +/- SEM) in comparison with group of patients with endemic nephropathy and the duration of disease less than 5 years (35.57 +/- 1.75), which was statistically significant (p < 0.03).
在60名年龄在30至60岁之间的地方性肾病患者（30名男性和30名女性）中，测量了病程对血清血管紧张素转换酶（ACE）的影响。分为三组：地方性肾病且病程小于5年的患者（n = 23）、地方性肾病且病程在5至10年之间的患者（n = 17）；以及地方性肾病且病程10年及以上的患者（n = 20）。采用分光光度法，以Hip-Gly-Gly为底物测定血清ACE活性。酶活性以每分钟每毫升血清水解Hip-Gly-Gly释放1 nmol马尿酸所对应的单位表示。结果显示，与地方性肾病且病程小于5年的患者组（35.57 +/- 1.75）相比，地方性肾病且病程10年及以上的患者组血清ACE活性降低（29.21 +/- 2.25；X +/- SEM），差异具有统计学意义（p < 0.03）。
