Camin F, Bontempo L, Heinrich K, Horacek M, Kelly S D, Schlicht C, Thomas F, Monahan F J, Hoogewerff J, Rossmann A
IASMA Research Center--Agrifood Quality Department, via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy.
Anal Bioanal Chem. 2007 Sep;389(1):309-20. doi: 10.1007/s00216-007-1302-3. Epub 2007 May 10.
Multi-element (H,C,N,S) stable isotope ratio analysis was tested for its suitability as a means for geographical provenance assignment of lamb meat from several European regions. The defatted dry matter (crude protein fraction) from lamb meat was found to be a suitable probe for "light" element stable isotope ratio analysis. Significant differences were observed between the multi-element isotope ratios of lamb samples from different regions. The mean hydrogen isotopic ratios of the defatted dry matter from lamb were found to be significantly correlated with the mean hydrogen isotopic ratios of precipitation and groundwater in the production regions. Carbon and nitrogen isotopic ratios were influenced by feeding practices and climate. Sulfur isotopic ratios were influenced by geographical location and surface geology of the production region. The results permitted differentiation of lamb meat, from most production regions, by inspection. However, more sophisticated evaluation of the data using multivariate methods, such as linear discriminant analysis, achieved 78% correct classification.
对多元素（氢、碳、氮、硫）稳定同位素比率分析作为确定来自欧洲几个地区的羔羊肉地理来源的一种方法的适用性进行了测试。发现羔羊肉的脱脂干物质（粗蛋白部分）是用于“轻”元素稳定同位素比率分析的合适样本。不同地区羔羊肉样本的多元素同位素比率之间存在显著差异。发现羔羊肉脱脂干物质的平均氢同位素比率与生产地区降水和地下水的平均氢同位素比率显著相关。碳和氮同位素比率受饲养方式和气候影响。硫同位素比率受生产地区地理位置和地表地质影响。通过检测，这些结果能够区分大多数生产地区的羔羊肉。然而，使用多变量方法（如线性判别分析）对数据进行更复杂的评估，正确分类率达到了78%。
