单纯疱疹病毒1型稳定的2千碱基潜伏相关转录本可改变60S核糖体亚基的组装，并通过依赖CRM1的途径从细胞核输出到细胞质。
The stable 2-kilobase latency-associated transcript of herpes simplex virus type 1 can alter the assembly of the 60S ribosomal subunit and is exported from nucleus to cytoplasm by a CRM1-dependent pathway.
作者信息
Atanasiu Doina, Fraser Nigel W
机构信息
Department of Microbiology, University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, PA 19104, USA.
出版信息
J Virol. 2007 Jul;81(14):7695-701. doi: 10.1128/JVI.00282-07. Epub 2007 May 9.
Abstract
During latency of herpes simplex virus type 1 in the neurons of the peripheral nervous system, the major transcript detected is the 2-kb latency-associated transcript (LAT) intron. During lytic infection, this intron has been shown to associate with ribosomes, suggesting a role in modifying the translational machinery of infected cells. In this study we show, using LAT-transfected cells, that the interaction of the intron with the 60S ribosomal subunit leads to irreversible changes in the sedimentation profile of this subunit in the nucleus. Furthermore, the 2-kb LAT intron is transported to the cytoplasm as part of the 60S ribosomal subunit, using a CRM1-dependent pathway.
摘要
在单纯疱疹病毒1型潜伏于外周神经系统神经元期间，检测到的主要转录本是2kb的潜伏相关转录本（LAT）内含子。在裂解感染期间，已证明该内含子与核糖体相关，提示其在改变受感染细胞翻译机制中发挥作用。在本研究中，我们利用转染了LAT的细胞表明，该内含子与60S核糖体亚基的相互作用导致该亚基在细胞核中的沉降图谱发生不可逆变化。此外，2kb的LAT内含子作为60S核糖体亚基的一部分，通过依赖CRM1的途径转运至细胞质。
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