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The stable 2-kilobase latency-associated transcript of herpes simplex virus type 1 can alter the assembly of the 60S ribosomal subunit and is exported from nucleus to cytoplasm by a CRM1-dependent pathway.单纯疱疹病毒1型稳定的2千碱基潜伏相关转录本可改变60S核糖体亚基的组装，并通过依赖CRM1的途径从细胞核输出到细胞质。
J Virol. 2007 Jul;81(14):7695-701. doi: 10.1128/JVI.00282-07. Epub 2007 May 9.
2
Herpes simplex virus type 1 2-kilobase latency-associated transcript intron associates with ribosomal proteins and splicing factors.1型单纯疱疹病毒2千碱基潜伏相关转录本内含子与核糖体蛋白和剪接因子相关联。
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The herpes simplex virus type 1 2.0-kilobase latency-associated transcript is a stable intron which branches at a guanosine.1型单纯疱疹病毒2.0千碱基潜伏相关转录本是一个在鸟苷处分支的稳定内含子。
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Construction of a herpes simplex virus type 1 mutant with only a three-nucleotide change in the branchpoint region of the latency-associated transcript (LAT) and the stability of its two-kilobase LAT intron.构建一种1型单纯疱疹病毒突变体，其潜伏相关转录本（LAT）分支点区域仅发生三个核苷酸的变化及其2千碱基LAT内含子的稳定性。
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本文引用的文献

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Ribosomal RNA processing and ribosome biogenesis in eukaryotes.真核生物中的核糖体RNA加工与核糖体生物合成
IUBMB Life. 2004 Aug;56(8):457-65. doi: 10.1080/15216540400010867.
2
Human cytomegalovirus 5-kilobase immediate-early RNA is a stable intron.人巨细胞病毒5千碱基立即早期RNA是一种稳定的内含子。
J Virol. 2004 Dec;78(23):13182-9. doi: 10.1128/JVI.78.23.13182-13189.2004.
3
CsdA, a cold-shock RNA helicase from Escherichia coli, is involved in the biogenesis of 50S ribosomal subunit.CsdA是一种来自大肠杆菌的冷休克RNA解旋酶，参与50S核糖体亚基的生物合成。
Nucleic Acids Res. 2004 May 17;32(9):2751-9. doi: 10.1093/nar/gkh603. Print 2004.
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Nuclear substructure and dynamics.核子结构与动力学。
Curr Biol. 2003 Oct 28;13(21):R825-8. doi: 10.1016/j.cub.2003.10.012.
5
The DEAD-box RNA helicase SrmB is involved in the assembly of 50S ribosomal subunits in Escherichia coli.DEAD盒RNA解旋酶SrmB参与大肠杆菌50S核糖体亚基的组装。
Mol Microbiol. 2003 Jun;48(5):1253-65. doi: 10.1046/j.1365-2958.2003.03513.x.
6
Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway.高等真核生物中核糖体亚基的生物合成及核输出依赖于CRM1输出途径。
J Cell Sci. 2003 Jun 15;116(Pt 12):2409-19. doi: 10.1242/jcs.00464. Epub 2003 Apr 30.
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The expanding snoRNA world.不断扩展的小核仁RNA世界
Biochimie. 2002 Aug;84(8):775-90. doi: 10.1016/s0300-9084(02)01402-5.
8
Nuclear export of ribosomal subunits.核糖体亚基的核输出。
Trends Biochem Sci. 2002 Nov;27(11):580-5. doi: 10.1016/s0968-0004(02)02208-9.
9
A protein encoded by the herpes simplex virus (HSV) type 1 2-kilobase latency-associated transcript is phosphorylated, localized to the nucleus, and overcomes the repression of expression from exogenous promoters when inserted into the quiescent HSV genome.由单纯疱疹病毒1型（HSV-1）2千碱基潜伏相关转录本编码的一种蛋白质被磷酸化，定位于细胞核，并且当插入静止的HSV基因组时可克服对外源启动子表达的抑制作用。
J Virol. 2002 Apr;76(8):4056-67. doi: 10.1128/jvi.76.8.4056-4067.2002.
10
The 2-kilobase intron of the herpes simplex virus type 1 latency-associated transcript has a half-life of approximately 24 hours in SY5Y and COS-1 cells.单纯疱疹病毒1型潜伏相关转录本的2千碱基内含子在SY5Y细胞和COS-1细胞中的半衰期约为24小时。
J Virol. 2002 Jan;76(2):532-40. doi: 10.1128/jvi.76.2.532-540.2002.

单纯疱疹病毒1型稳定的2千碱基潜伏相关转录本可改变60S核糖体亚基的组装，并通过依赖CRM1的途径从细胞核输出到细胞质。

The stable 2-kilobase latency-associated transcript of herpes simplex virus type 1 can alter the assembly of the 60S ribosomal subunit and is exported from nucleus to cytoplasm by a CRM1-dependent pathway.

作者信息

Atanasiu Doina, Fraser Nigel W

机构信息

Department of Microbiology, University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, PA 19104, USA.

出版信息

J Virol. 2007 Jul;81(14):7695-701. doi: 10.1128/JVI.00282-07. Epub 2007 May 9.

DOI:10.1128/JVI.00282-07
PMID:17494083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1933333/
Abstract

During latency of herpes simplex virus type 1 in the neurons of the peripheral nervous system, the major transcript detected is the 2-kb latency-associated transcript (LAT) intron. During lytic infection, this intron has been shown to associate with ribosomes, suggesting a role in modifying the translational machinery of infected cells. In this study we show, using LAT-transfected cells, that the interaction of the intron with the 60S ribosomal subunit leads to irreversible changes in the sedimentation profile of this subunit in the nucleus. Furthermore, the 2-kb LAT intron is transported to the cytoplasm as part of the 60S ribosomal subunit, using a CRM1-dependent pathway.

摘要

在单纯疱疹病毒1型潜伏于外周神经系统神经元期间，检测到的主要转录本是2kb的潜伏相关转录本（LAT）内含子。在裂解感染期间，已证明该内含子与核糖体相关，提示其在改变受感染细胞翻译机制中发挥作用。在本研究中，我们利用转染了LAT的细胞表明，该内含子与60S核糖体亚基的相互作用导致该亚基在细胞核中的沉降图谱发生不可逆变化。此外，2kb的LAT内含子作为60S核糖体亚基的一部分，通过依赖CRM1的途径转运至细胞质。