一只自由放养的驼鹿（北美驼鹿指名亚种）患慢性消耗病的自然病例。

A natural case of chronic wasting disease in a free-ranging moose (Alces alces shirasi).

作者信息

Baeten Laurie A, Powers Barbara E, Jewell Jean E, Spraker Terry R, Miller Michael W

机构信息

Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, CO 80526-2097, USA.

出版信息

J Wildl Dis. 2007 Apr;43(2):309-14. doi: 10.7589/0090-3558-43.2.309.

DOI:10.7589/0090-3558-43.2.309

PMID:17495319

Abstract

Chronic wasting disease (CWD) was diagnosed in a free-ranging moose (Alces alces shirasi) killed by a hunter in Jackson County, Colorado, USA, in September 2005. The diagnosis was based upon immunohistochemistry (IHC) demonstrating the presence of accumulations of CWD-associated prion protein (PrP(CWD)) in tissue sections of medulla oblongata at the level of the obex (dorsal motor nucleus of the vagus) and in retropharyngeal lymph node (RPLN); additional testing by IHC revealed deposits of PrP(CWD) in multiple sections of medulla oblongata and cervical spinal cord as well as palatine tonsil and submandibular lymph node tissues. Western blot confirmed the presence of PrP(CWD) in RPLN and tonsil tissue. The PrP(CWD) also was detected via enzyme-linked immunosorbent assay of RPLN tissue. Spongiform encephalopathy was observed in sections of the brainstem and cervical spinal cord, although no clinical signs were noted by the hunter who killed the animal. The affected moose was homozygous for methionine at codon 209 of the prion protein coding region. In October 2006, two additional free-ranging moose were diagnosed with CWD. Epidemiology and implications of CWD in moose remain to be determined.

摘要

2005年9月，在美国科罗拉多州杰克逊县，一头被猎人猎杀的野生驼鹿（北美驼鹿指名亚种）被诊断出患有慢性消耗病（CWD）。诊断依据是免疫组织化学（IHC）显示，在延髓闩部（迷走神经背运动核）水平的组织切片以及咽后淋巴结（RPLN）中存在与CWD相关的朊病毒蛋白（PrP(CWD)）聚集；IHC的进一步检测显示，在延髓和颈脊髓的多个切片以及腭扁桃体和下颌下淋巴结组织中存在PrP(CWD)沉积。蛋白质印迹法证实RPLN和扁桃体组织中存在PrP(CWD)。通过对RPLN组织进行酶联免疫吸附测定也检测到了PrP(CWD)。在脑干和颈脊髓切片中观察到海绵状脑病，尽管杀死这头动物的猎人未注意到任何临床症状。受影响的驼鹿在朊病毒蛋白编码区第209密码子处为甲硫氨酸纯合子。2006年10月，另外两头野生驼鹿被诊断出患有CWD。CWD在驼鹿中的流行病学及影响仍有待确定。