Kreeger Terry J, Montgomery D L, Jewell Jean E, Schultz Will, Williams Elizabeth S
Wyoming Game and Fish Department, 2362 Highway 34, Wheatland, Wyoming 82201, USA.
J Wildl Dis. 2006 Jul;42(3):640-5. doi: 10.7589/0090-3558-42.3.640.
Three captive Shira's moose (Alces alces shirasi) were orally inoculated with a single dose (5 g) of whole-brain homogenate prepared from chronic wasting disease (CWD)-affected mule deer (Odocoileus hemionus). All moose died of causes thought to be other than CWD. Histologic examination of one female moose dying 465 days postinoculation revealed spongiform change in the neuropil, typical of transmissible spongiform encephalopathy. Immunohistochemistry staining for the proteinase-resistant isoform of the prion protein was observed in multiple lymphoid and nervous tissues. Western blot and enzyme-linked immunosorbent assays provided additional confirmation of CWD. These results represent the first report of experimental CWD in moose.
三只圈养的希拉驼鹿（Alces alces shirasi）经口接种了一剂（5克）由患慢性消耗病（CWD）的骡鹿（Odocoileus hemionus）制备的全脑匀浆。所有驼鹿均死于被认为并非由CWD导致的原因。对一只在接种后465天死亡的雌性驼鹿进行的组织学检查显示，神经纤维网出现海绵状变化，这是传染性海绵状脑病的典型特征。在多个淋巴组织和神经组织中观察到了针对朊病毒蛋白抗蛋白酶异构体的免疫组织化学染色。蛋白质印迹法和酶联免疫吸附测定进一步证实了CWD。这些结果代表了驼鹿实验性CWD的首次报告。
