Circella Elena, Camarda Antonio, Martella Vito, Bruni Giordano, Lavazza Antonio, Buonavoglia Canio
Dipartimento di Sanità e Benessere degli Animali, Università degli Studi di Bari, S.P. Casamassima Km 3, Facoltà di Medicina Veterinaria, Valenzano, Bari, Italy.
Avian Pathol. 2007 Jun;36(3):251-8. doi: 10.1080/03079450701344738.
An enteric syndrome was observed in quail (Coturnix coturnix) semi-intensively reared for restocking in Apulia (southern Italy). The birds showed depression, severe diarrhoea, dehydration and reduced growth. Mortality occurred particularly in young birds. At necropsy the prominent lesion was enteritis. A coronavirus was detected by electron microscopy and reverse transcriptase-polymerase chain reaction in the faeces and in the intestinal content of the dead quails. The virus could not be cultivated in chicken embryos. By sequence analyses of a fragment (409 nucleotides) of region 1b of the polymerase gene, the quail coronavirus displayed <or=93% nucleotide identity to avian coronaviruses (group 3 coronaviruses)--whereas by analysis of the S1 portion of the spike protein-encoding gene, the quail coronavirus displayed 16% to 18% amino acid identity with infectious bronchitis virus, and 79% to 81% identity with turkey coronavirus. Altogether, the findings suggest the existence of a novel coronavirus genetically related to turkey coronavirus.
在意大利南部普利亚大区用于补栏的半集约化饲养鹌鹑（日本鹌鹑）中观察到一种肠道综合征。这些鹌鹑表现出精神沉郁、严重腹泻、脱水和生长迟缓。死亡尤其发生在幼鸟中。尸检时突出病变为肠炎。通过电子显微镜和逆转录 - 聚合酶链反应在死亡鹌鹑的粪便和肠道内容物中检测到一种冠状病毒。该病毒不能在鸡胚中培养。通过对聚合酶基因1b区域的一个片段（409个核苷酸）进行序列分析，鹌鹑冠状病毒与禽冠状病毒（第3组冠状病毒）的核苷酸同一性≤93%；而通过对编码刺突蛋白的基因的S1部分进行分析，鹌鹑冠状病毒与传染性支气管炎病毒的氨基酸同一性为16%至18%，与火鸡冠状病毒的同一性为79%至81%。总体而言，这些发现表明存在一种与火鸡冠状病毒有遗传关系的新型冠状病毒。
