Zeng Jia-wei, Wang Shi-xin, Zhao Xue-feng, Wei Mao-ti, Tu Zhi-guang
Department of Laboratory Medicine, Chongqing University of Medical Science, Chongqing 400016, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Mar;25(3):136-41.
To establish 2-dimensional gel electrophoresis (2-DE) images and seek differentially expressed serum proteins for understanding the pathogenesis of silicosis.
2-DE and matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF-MS/MS) were used to screen differentially expressed serum proteins among silica-exposed population, suspect of silicosis (0+), phase one (I) group with silicosis and control group(non silica exposure).
Complement C4, leucine-rich alpha-2-glycoprotein and alpha-1-antitrypsin were significantly highly expressed in suspect of silicosis (0+) group(P < 0.01), but lowly in other groups. Inversely, serotransferrin was significantly down-regulated only in suspect of silicosis (0+) group(P < 0.01). Plasma glutathione peroxidase, tetranectin, apolipoprotein A-I and transthyretin were equally expressed in the serum of control group and silica-exposed population group, but decreased in the suspect of silicosis (0+) and phase (I) group.
Complement C4, leucine-rich alpha-2-glycoprotein, alpha-1-antitrypsin, serotransferrin, plasma glutathione peroxidase, tetranectin, apolipoprotein A-I and transthyretin are differentially expressed in the silica-exposed group and phase (I) group with silicosis, and the result should be validated by other biochemical technologies.
建立二维凝胶电泳（2-DE）图像并寻找差异表达的血清蛋白，以了解矽肺的发病机制。
采用二维凝胶电泳和基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF-MS/MS）技术，筛选矽尘暴露人群、疑似矽肺（0+）组、一期（I）矽肺组和对照组（非矽尘暴露）之间差异表达的血清蛋白。
补体C4、富含亮氨酸的α-2-糖蛋白和α-1-抗胰蛋白酶在疑似矽肺（0+）组中显著高表达（P<0.01），而在其他组中低表达。相反，转铁蛋白仅在疑似矽肺（0+）组中显著下调（P<0.01）。血浆谷胱甘肽过氧化物酶、四连蛋白、载脂蛋白A-I和转甲状腺素蛋白在对照组和矽尘暴露人群组血清中表达水平相当，但在疑似矽肺（0+）组和一期（I）组中降低。
补体C4、富含亮氨酸的α-2-糖蛋白、α-1-抗胰蛋白酶、转铁蛋白、血浆谷胱甘肽过氧化物酶、四连蛋白、载脂蛋白A-I和转甲状腺素蛋白在矽尘暴露组和一期（I）矽肺组中差异表达，该结果应通过其他生化技术进行验证。
