Jiang Hong-Yan, Wang Shi-Xin, Li Xue-Hua, Wei Mao-Ti, Yang Xia, Chen Juan-Juan, Luan Da-Wei, Li Zhe, Chen Yi-Hui, Weng Ya-Guang
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Jul;42(7):522-6.
To seek differentially expressed serum proteins in recovered SARS patients complicating avascular necrosis of femoral head (AVNFH).
2-DE and MALDI-TOF MS were used to study the comparative serum proteomics among female SARS AVNFH group, female SARS non-AVNFH group and female healthy group. ELISA method was used to detect serum amyloid P component in individual serum; specificity and sensitivity of serum amyloid P component were analyzed.
Average protein points on 2-DE of 3 groups were 632 +/- 28, 671 +/- 55, 688 +/- 42 respectively, and the matching rate of protein points was ranged from 85% to 95%; eighteen differentially expressed proteins were discovered including transthyretin, serpin peptidase inhibitor, alpha-1-antitrypsin precursor, serum amyloid P components, etc. Compared to healthy group and SARS non-AVNFH group, transthyretin, C4B3, fibrinogen gamma, apolipoprotein L, apolipoprotein A-IV precursor, albumin and prealbumin showed lower expression, inversely serpin peptidase inhibitor, alpha-1-antitrypsin precursor and serum amyloid P components showed higher expression in serum in the SARS AVNFH necrosis group. The serum amyloid P component in 3 groups were 0.54 +/- 0.30 ng/ml, 0.83 +/- 0.39 ng/ml, 1.21 +/- 0.29 ng/ml respectively. The areas under the ROC curve on serum amyloid P component was 0.854, the specificity was 77.8% and the sensitivity was 85.2%.
There were differentially expressed serum proteins in three groups. Serum amyloid P components might be one of the potential biomarkers in serum of recovered SARS patients complicating avascular necrosis of femoral head.
寻找康复期并发股骨头缺血性坏死（AVNFH）的非典患者血清中差异表达的蛋白质。
采用二维电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，对女性非典并发AVNFH组、女性非典未并发AVNFH组和女性健康组进行血清蛋白质组学比较研究。采用酶联免疫吸附测定（ELISA）法检测个体血清中的血清淀粉样蛋白P成分；分析血清淀粉样蛋白P成分的特异性和敏感性。
三组二维电泳平均蛋白点数分别为632±28、671±55、688±42，蛋白点匹配率在85%至95%之间；发现18种差异表达蛋白，包括转甲状腺素蛋白、丝氨酸蛋白酶抑制剂、α-1抗胰蛋白酶前体、血清淀粉样蛋白P成分等。与健康组和非典未并发AVNFH组相比，转甲状腺素蛋白、C4B3、纤维蛋白原γ、载脂蛋白L、载脂蛋白A-IV前体、白蛋白和前白蛋白在非典并发AVNFH坏死组血清中表达较低，相反，丝氨酸蛋白酶抑制剂、α-1抗胰蛋白酶前体和血清淀粉样蛋白P成分在血清中表达较高。三组血清淀粉样蛋白P成分分别为0.54±0.30 ng/ml、0.83±0.39 ng/ml、1.21±0.29 ng/ml。血清淀粉样蛋白P成分的ROC曲线下面积为0.854，特异性为77.8%，敏感性为85.2%。
三组存在差异表达的血清蛋白。血清淀粉样蛋白P成分可能是康复期并发股骨头缺血性坏死的非典患者血清中的潜在生物标志物之一。
