Li Hengde, Xu Shangzhong, Gao Xue, Ren Hongyan
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
J Genet Genomics. 2007 Apr;34(4):315-20. doi: 10.1016/S1673-8527(07)60033-2.
Acyl-coenzyme A dehydrogenases (ACAD) are a family of nuclear-coded, mitochondrial flavoenzymes that catalyze the alpha, and beta-dehydrogenation of fatty acids. The eighth member of this family, ACAD8 catalyzes the valine catabolism. In this study, the bovine ACAD8 full-length mRNA and genomic DNA sequence were obtained and its gene structure was determined through alignment of the genomic DNA sequence to the mRNA sequence. The mRNA sequence consisted of a 1,251 bp open reading frame (ORF) flanked by a 37 bp 5'-untranslated region (UTR) and a 444 bp 3'-UTR; and its full-length genomic DNA sequence was 13,814 bp in length and included 11 exons and 10 introns. One A-G single nucleotide polymorphism (SNP) was revealed at nucleotide 13,408 (GenBank accession No. DQ435445) in the bovine ACAD8 gene by sequencing the polymerase chain reaction (PCR) products of 6 randomly selected individuals from the sample population. Different genotypes were determined by restriction fragment length polymorphism (RFLP). The association analysis of this SNP in bovine ACAD8 with production traits in 178 unrelated steers from 5 breeds showed that it had a significant effect on the daily gain and the beef tenderness (P < 0.05). Cattle with the G allele grew more rapidly and the beef they produced was more tender than those with the A allele. Thus, this SNP of the bovine ACAD8 gene can be used as an indicator to improve the growth rate and the beef tenderness.
酰基辅酶A脱氢酶（ACAD）是一族由细胞核编码的线粒体黄素酶，可催化脂肪酸的α和β脱氢反应。该家族的第八个成员ACAD8催化缬氨酸分解代谢。在本研究中，获得了牛ACAD8全长mRNA和基因组DNA序列，并通过将基因组DNA序列与mRNA序列比对确定了其基因结构。mRNA序列由一个1251 bp的开放阅读框（ORF）组成，两侧分别是一个37 bp的5'非翻译区（UTR）和一个444 bp的3'UTR；其全长基因组DNA序列长度为13814 bp，包含11个外显子和10个内含子。通过对从样本群体中随机选取的6个个体的聚合酶链反应（PCR）产物进行测序，在牛ACAD8基因的第13408位核苷酸处（GenBank登录号：DQ435445）发现了一个A-G单核苷酸多态性（SNP）。通过限制性片段长度多态性（RFLP）确定了不同的基因型。对来自5个品种的178头非亲缘公牛中该牛ACAD8基因SNP与生产性状的关联分析表明，它对日增重和牛肉嫩度有显著影响（P < 0.05）。具有G等位基因的牛生长速度更快，其生产的牛肉比具有A等位基因的牛更嫩。因此，牛ACAD8基因的这个SNP可作为提高生长速度和牛肉嫩度的一个指标。
