Zhang Sihuan, Dang Yonglong, Zhang Qingfeng, Qin Qiaomei, Lei Chuzhao, Chen Hong, Lan Xianyong
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Gene. 2015 Apr 1;559(2):184-8. doi: 10.1016/j.gene.2015.01.043. Epub 2015 Jan 22.
Acyl-CoA dehydrogenase, very long chain (ACADVL), encoding ACADVL protein, targets the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway and plays an important role in body metabolism and oxidation of long chain fatty acid releasing energy. Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is an easy-to-operate, rapid, inexpensive, and exact method for SNP genotyping. Herein, T-ARMS-PCR was carried out to detect a critical missense mutation (AC_000176:g.2885C>A; Pro236Thr) within the ACADVL gene in 644 individuals from two cattle breeds. In order to evaluate the accuracy of the T-ARMS-PCR at this locus, the genotype of the sampled individuals was also identified by PCR-RFLP. The concordance between these two methods was 98.76%. Statistical analysis showed that the bovine ACADVL gene had a significant effect on chest width (P<0.05), chest depth (P<0.05), and hip width (P<0.05) in the Qinchuan breed. The cattle with AA genotype had superior growth traits compared to cattle with AC and/or CC genotypes. The "A" allele had positive effects on growth traits. Therefore, T-ARMS-PCR can replace PCR-RFLP for rapid genotyping of this mutation, which could be used as a DNA marker for selecting individuals with superior growth traits in the Qinchuan breed. These findings contribute to breeding and genetics in beef cattle industry.
酰基辅酶A脱氢酶超长链（ACADVL）编码ACADVL蛋白，定位于线粒体内膜，在那里催化线粒体脂肪酸β-氧化途径的第一步，在机体代谢以及长链脂肪酸氧化释放能量过程中发挥重要作用。四引物扩增阻滞突变系统PCR（T-ARMS-PCR）是一种操作简便、快速、廉价且准确的单核苷酸多态性（SNP）基因分型方法。在此，运用T-ARMS-PCR对两个牛品种的644个个体的ACADVL基因中的一个关键错义突变（AC_000176:g.2885C>A；Pro236Thr）进行检测。为评估T-ARMS-PCR在该位点的准确性，还通过PCR-RFLP对采样个体的基因型进行鉴定。这两种方法的一致性为98.76%。统计分析表明，秦川牛品种中，牛ACADVL基因对胸围（P<0.05）、胸深（P<0.05）和臀围（P<0.05）有显著影响。与AC和/或CC基因型的牛相比，AA基因型的牛具有更优的生长性状。“A”等位基因对生长性状有积极影响。因此，T-ARMS-PCR可替代PCR-RFLP对该突变进行快速基因分型，其可作为一个DNA标记用于选择秦川牛品种中具有优良生长性状的个体。这些研究结果对肉牛产业的育种和遗传学研究有重要意义。
