Ray Subhasree, Jin Jun-O, Choi Inho, Kim Myunghee
Research Institute of Cell Culture, Yeungnam University, Gyeongsan, South Korea.
Department of Life Science, School of Basic Science and Research, Sharda University, Greater Noida, India.
Front Bioeng Biotechnol. 2023 Jan 6;10:907500. doi: 10.3389/fbioe.2022.907500. eCollection 2022.
Growing concerns over the use of limited fossil fuels and their negative impacts on the ecological niches have facilitated the exploration of alternative routes. The use of conventional plastic material also negatively impacts the environment. One such green alternative is polyhydroxyalkanoates, which are biodegradable, biocompatible, and environmentally friendly. Recently, researchers have focused on the utilization of waste gases particularly those belonging to C1 sources derived directly from industries and anthropogenic activities, such as carbon dioxide, methane, and methanol as the substrate for polyhydroxyalkanoates production. Consequently, several microorganisms have been exploited to utilize waste gases for their growth and biopolymer accumulation. Methylotrophs such as produced highest amount of PHA up to 88% using CH as the sole carbon source and 52-56% with CHOH. On the other hand , produced 71-81% of PHA by utilizing CO and CO as a substrate. The present review shows the potential of waste gas valorization as a promising solution for the sustainable production of polyhydroxyalkanoates. Key bottlenecks towards the usage of gaseous substrates obstructing their realization on a large scale and the possible technological solutions were also highlighted. Several strategies for PHA production using C1 gases through fermentation and metabolic engineering approaches are discussed. Microbes such as autotrophs, acetogens, and methanotrophs can produce PHA from CO, CO, and CH. Therefore, this article presents a vision of C1 gas into bioplastics are prospective strategies with promising potential application, and aspects related to the sustainability of the system.
对有限化石燃料使用及其对生态位负面影响的日益关注促使人们探索替代途径。传统塑料材料的使用也对环境产生负面影响。一种这样的绿色替代品是聚羟基脂肪酸酯，它具有生物可降解性、生物相容性且环境友好。最近，研究人员专注于利用废气，特别是那些直接来自工业和人为活动的C1源废气，如二氧化碳、甲烷和甲醇作为聚羟基脂肪酸酯生产的底物。因此，已经开发了几种微生物来利用废气进行生长和生物聚合物积累。甲基营养菌如在以CH作为唯一碳源时可产生高达88%的聚羟基脂肪酸酯，以CHOH为碳源时可产生52 - 56%。另一方面，通过利用CO和CO作为底物可产生71 - 81%的聚羟基脂肪酸酯。本综述展示了废气增值作为聚羟基脂肪酸酯可持续生产的一种有前景解决方案的潜力。还强调了阻碍气态底物大规模应用的关键瓶颈以及可能的技术解决方案。讨论了通过发酵和代谢工程方法使用C1气体生产聚羟基脂肪酸酯的几种策略。自养菌、产乙酸菌和甲烷氧化菌等微生物可以从CO、CO和CH生产聚羟基脂肪酸酯。因此，本文提出了将C1气体转化为生物塑料是具有潜在应用前景的前瞻性策略以及与该系统可持续性相关的方面。
