Bittencourt C S, Azzolini A E C S, Ferreira D A, Assis-Pandochi A I
Depto. de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14040903, Brazil.
Int Immunopharmacol. 2007 Jul;7(7):989-93. doi: 10.1016/j.intimp.2007.02.016. Epub 2007 Mar 30.
This study evaluated antibody production against sheep red blood cells (SRBC) in hyperthyroid rats during treatment with triiodothyronine (T(3)). The immune response was evaluated by measuring plaque forming cells (PFC) in the spleen and by enzyme-linked immunosorbent assay (ELISA) in serum of male Wistar rats (180+/-10 g) treated with 25 mug/day of triiodotironine (T(3)) during 7-12 days and immunized with SRBC at the 8th day of treatment. The results showed that anti-SRBC antibody production was significantly decreased in animals treated for 12 days when compared to normal rats immunized with the same antigen, as evaluated by the two assays. These results show that in this experimental model hyperthyroidism decreases antibody response. We previously observed the opposite effect, that is, an increase in this response in hypothyroid rats resulting from the treatment with propylthyouracil, a blocker of thyroid hormone biosynthesis. It is suggested that antibody production is affected by thyroid hormone levels.
本研究评估了甲状腺功能亢进大鼠在接受三碘甲状腺原氨酸（T₃）治疗期间针对绵羊红细胞（SRBC）的抗体产生情况。通过测量雄性Wistar大鼠（180±10克）脾脏中的空斑形成细胞（PFC）以及在治疗7 - 12天期间每天给予25微克三碘甲状腺原氨酸（T₃）并在治疗第8天用SRBC免疫的大鼠血清中的酶联免疫吸附测定（ELISA）来评估免疫反应。结果表明，与用相同抗原免疫的正常大鼠相比，经两种测定评估，接受12天治疗的动物中抗SRBC抗体产生显著降低。这些结果表明，在该实验模型中甲状腺功能亢进会降低抗体反应。我们之前观察到相反的效果，即丙硫氧嘧啶（一种甲状腺激素生物合成阻滞剂）治疗导致的甲状腺功能减退大鼠中这种反应增加。提示抗体产生受甲状腺激素水平影响。
