Deloménie Claudine, Foti Emilie, Floch Enora, Diderot Vimala, Porquet Dominique, Dupuy Corinne, Bonaly Jacqueline
IFR 141, Plateforme Transcriptome, Faculté de Pharmacie, Université Paris-Sud, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry, France.
Biochem Biophys Res Commun. 2007 Jun 29;358(2):455-61. doi: 10.1016/j.bbrc.2007.04.145. Epub 2007 May 2.
To better understand the cellular mechanism of stress resistance to various pollutants (cadmium, pentachlorophenol), we undertook a survey of the Euglena gracilis transcriptome by mRNA differential display and cDNA cloning. We performed a real-time RT-PCR analysis upon four selected genes. One of them significantly changed its expression level in response to stress treatments: B25 gene was overexpressed in Cd-resistant cells whereas it was down-regulated in PCP-adapted cells. By Race assays we obtained for B25 a 1093bp cDNA. The deduced protein was identified as a bacterial formate/nitrite transporter (FocA) homolog and the gene was named EgFth. From all the data, we concluded that EgFth overexpression was related to chronic exposure to cadmium.
为了更好地理解纤细裸藻对各种污染物（镉、五氯苯酚）的抗逆细胞机制，我们通过mRNA差异显示和cDNA克隆对纤细裸藻转录组进行了调查。我们对四个选定基因进行了实时RT-PCR分析。其中一个基因在应激处理后其表达水平发生了显著变化：B25基因在抗镉细胞中过表达，而在适应五氯苯酚的细胞中被下调。通过RACE分析，我们获得了B25的一个1093bp的cDNA。推导的蛋白质被鉴定为细菌甲酸/亚硝酸盐转运蛋白（FocA）同源物，该基因被命名为EgFth。从所有数据中，我们得出结论，EgFth的过表达与长期接触镉有关。
