Barque J P, Chacun H, Marouby S, Bonaly J
Laboratoire Métabolisme Cellulaire et Xénobiotiques, Faculté de Pharmacie, Châtenay-Malabry, France.
Biochem Biophys Res Commun. 1994 Aug 30;203(1):540-4. doi: 10.1006/bbrc.1994.2216.
The heat-shock response of Euglena gracilis was studied by cell labeling at both the normal growth temperature (23 degrees C) and an elevated temperature (35 degrees C). Analysis of the labeled proteins by two-dimensional polyacrylamide gel electrophoresis indicated that the rate of synthesis of two polypeptides p55 (55 kDa) and p40 (40 kDa) increased in cells labeled at the highest temperature studied. These polypeptides are also overexpressed in Cadmium-resistant Euglena gracilis cells labeled at the normal growth temperature (23 degrees C). On the basis of these results, p55 and p40 appear to be heat-shock proteins involved in some steps of the acquired Cd-resistance process in Euglena gracilis cells.
通过在正常生长温度（23摄氏度）和升高温度（35摄氏度）下对细胞进行标记，研究了纤细裸藻的热休克反应。通过二维聚丙烯酰胺凝胶电泳对标记蛋白进行分析表明，在研究的最高温度下标记的细胞中，两种多肽p55（55 kDa）和p40（40 kDa）的合成速率增加。这些多肽在正常生长温度（23摄氏度）下标记的耐镉纤细裸藻细胞中也过表达。基于这些结果，p55和p40似乎是参与纤细裸藻细胞获得性镉抗性过程某些步骤的热休克蛋白。
