环境烟草烟雾对恒河猴精子功能的体外影响。

Effects of environmental tobacco smoke in vitro on rhesus monkey sperm function.

作者信息

Hung Pei Hsuan, Baumber Julie, Meyers Stuart A, VandeVoort Catherine A

机构信息

Molecular, Cellular and Integrative Physiology, University of California, Davis, CA 95616, USA.

出版信息

Reprod Toxicol. 2007 Jun;23(4):499-506. doi: 10.1016/j.reprotox.2007.03.005. Epub 2007 Apr 5.

DOI:10.1016/j.reprotox.2007.03.005

PMID:17499478

Abstract

The objective of this study was to use a non-human primate model to examine the effect of ETS on sperm function. Sperm samples were collected from adult rhesus monkeys (Macaca mulatta) and treated with different levels of ETS exposed medium. ETS treatment decreased the percentage of motile sperm and motion parameters. Sperm treated with ETS exposed medium showed a limited response to the activators and exhibited decreased binding to the zonae pellucida after activation. The mitochondrial integrity of the ETS-treated sperm was disrupted; however, there was no decrease in viability compared to control groups. Sperm acrosomal status was similar in the control and treatment groups. The results imply that the exposure of primate sperm to ETS could impair sperm transport in vivo.

摘要

本研究的目的是使用非人灵长类动物模型来研究环境烟草烟雾（ETS）对精子功能的影响。从成年恒河猴（猕猴）采集精子样本，并用不同水平的暴露于ETS的培养基进行处理。ETS处理降低了活动精子的百分比和运动参数。用暴露于ETS的培养基处理的精子对激活剂的反应有限，并且在激活后与透明带的结合减少。ETS处理的精子的线粒体完整性受到破坏；然而，与对照组相比，活力没有降低。对照组和处理组的精子顶体状态相似。结果表明，灵长类动物精子暴露于ETS可能会损害体内的精子运输。