Ghaffari Mohammad Ali, Rostami Morad
Physiology Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran ; Cellular and Molecular Research Center, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran ; Department of Biochemistry, School of Medicine, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran.
J Reprod Infertil. 2012 Apr;13(2):81-7.
Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as nitric oxide synthases. Low concentrations of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. Moreover, sperm membrane contains high concentrations of polyunsaturated fatty acids that are highly susceptible to oxidative damage that interferes with fertilization ability. Therefore, we investigated the correlation between levels of sperm malondialdehyde (MDA) and NO with sperm motility in male smokers.
Semen samples were collected from normozoospermic smoker (n=64) and nonsmoker (n=83) men. The content of sperm lipid peroxidation was determined by measuring malondialdehyde (MDA). The sperm NO were also measured using Griess reagent. Data was analyzed by SPSS, (version 15.0), using independent t-test and Pearson analysis.
The mean MDA and NO concentrations in the sperm of normozoospermic male smokers were significantly higher than the control group or normozoospermic nonsmokers, (p <0.001). A significant negative relationship was noted between sperm motility and sperm MDA levels (r=-0.32, p=0.01); and sperm motility and sperm NO concentration (for nitrite, r=-0.34, p=0.006 and for nitrate, r=-0.38, p=0.002).
It was concluded that the increase in MDA and NO production in sperm can influence sperm motility in normozoospermic smokers. Therefore, it seems that cigarette smoking may affect the fertility of male smokers via increasing the amount of sperm MDA/lipid peroxidation and NO concentrations.
一氧化氮（NO）由一组称为一氧化氮合酶的酶从L-精氨酸合成。低浓度的NO在精子的生物学和生理学中至关重要，但高浓度的NO具有毒性，会对精子功能产生负面影响。此外，精子膜含有高浓度的多不饱和脂肪酸，极易受到氧化损伤，从而干扰受精能力。因此，我们研究了男性吸烟者精子丙二醛（MDA）和NO水平与精子活力之间的相关性。
从精子正常的吸烟者（n = 64）和非吸烟者（n = 83）男性中收集精液样本。通过测量丙二醛（MDA）来确定精子脂质过氧化的含量。还使用格里斯试剂测量精子中的NO。数据采用SPSS（版本15.0）进行分析，使用独立样本t检验和Pearson分析。
精子正常的男性吸烟者精子中的平均MDA和NO浓度显著高于对照组或精子正常的非吸烟者（p <0.001）。精子活力与精子MDA水平之间存在显著负相关（r = -0.32，p = 0.01）；精子活力与精子NO浓度之间也存在显著负相关（亚硝酸盐，r = -0.34，p = 0.006；硝酸盐，r = -0.38，p = 0.002）。
得出的结论是，精子中MDA和NO生成的增加会影响精子正常的吸烟者的精子活力。因此，吸烟似乎可能通过增加精子MDA/脂质过氧化量和NO浓度来影响男性吸烟者的生育能力。
