Verdeil Jean-Luc, Alemanno Laurence, Niemenak Nicolas, Tranbarger Timothy John
Centre de Coopération Internationale en Recherche Agronomique pour le Développement, CIRAD, UMR 1096, TA 96/02, Montpellier Cedex 5, France.
Trends Plant Sci. 2007 Jun;12(6):245-52. doi: 10.1016/j.tplants.2007.04.002. Epub 2007 May 11.
Little is known of the mechanisms that induce the dedifferentiation of a single somatic cell into a totipotent embryogenic cell that can either be regenerated or develop into an embryo and subsequently an entire plant. In this Opinion article, we examine the cellular, physiological and molecular similarities and differences between different plant stem cell types. We propose to extend the plant stem cell concept to include single embryogenic cells as a totipotent stem cell based on their capacity to regenerate or develop into an embryo under certain conditions. Our survey suggests that differences in chromatin structure might ensure that meristem-localized stem cells have supervised freedom and are pluripotent, and that embryogenic stem cells are unsupervised, autonomous and, hence, freely totipotent.
关于诱导单个体细胞去分化为全能胚胎发生细胞的机制，人们了解甚少。这种全能胚胎发生细胞既可以再生，也可以发育成胚胎，随后发育成完整的植株。在这篇观点文章中，我们研究了不同植物干细胞类型之间在细胞、生理和分子层面的异同。基于单个胚胎发生细胞在特定条件下具有再生或发育成胚胎的能力，我们建议将植物干细胞的概念扩展到包括单个胚胎发生细胞作为全能干细胞。我们的研究表明，染色质结构的差异可能确保分生组织定位的干细胞受到调控，具有多能性，而胚胎发生干细胞则不受调控、自主，因此具有完全的全能性。
