Li Yaning, Uhm Taesik, Ren Chengwei, Wu Chengcang, Santos Teofila S, Lee Mi-Kyung, Yan Bo, Santos Felipe, Zhang Aimin, Scheuring Chantel, Sanchez Alma, Millena Anna C, Nguyen Henry T, Kou Hongda, Liu Daqun, Zhang Hong-Bin
Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA.
Genome. 2007 Mar;50(3):278-88. doi: 10.1139/g07-006.
Sequencing of the rice genome has provided a platform for functional genomics research of rice and other cereal species. However, multiple approaches are needed to determine the functions of its genes and sequences and to use the genome sequencing results for genetic improvement of cereal crops. Here, we report a plant-transformation-competent, binary bacterial artificial chromosome (BIBAC) and bacterial artificial chromosome (BAC) based map of rice to facilitate these studies. The map was constructed from 20 835 BIBAC and BAC clones, and consisted of 579 overlapping BIBAC/BAC contigs. To facilitate functional analysis of chromosome 8 genomic sequence and cloning of the genes and QTLs mapped to the chromosome, we anchored the chromosomal contigs to the existing rice genetic maps. The chromosomal map consists of 11 contigs, 59 genetic markers, and 36 sequence tagged sites, spanning a total of ca. 38 Mb in physical length. Comparative analysis between the genetic and physical maps of chromosome 8 showed that there are 3 "hot" and 2 "cold" spots of genetic recombination along the chromosomal arms in addition to the "cold spot" in the centromeric region, suggesting that the sequence component contents of a chromosome may affect its local genetic recombination frequencies. Because of its plant transformability, the BIBAC/BAC map could provide a platform for functional analysis of the rice genome sequence and effective use of the sequencing results for gene and QTL cloning and molecular breeding.
水稻基因组测序为水稻及其他谷类作物的功能基因组学研究提供了一个平台。然而，需要多种方法来确定其基因和序列的功能，并将基因组测序结果用于谷类作物的遗传改良。在此，我们报道了一种基于植物可转化的二元细菌人工染色体（BIBAC）和细菌人工染色体（BAC）的水稻图谱，以促进这些研究。该图谱由20835个BIBAC和BAC克隆构建而成，包含579个重叠的BIBAC/BAC重叠群。为便于对第8号染色体的基因组序列进行功能分析以及克隆定位到该染色体上的基因和数量性状基因座（QTL），我们将染色体重叠群锚定到现有的水稻遗传图谱上。该染色体图谱由11个重叠群、59个遗传标记和36个序列标签位点组成，物理长度总共约为38 Mb。第8号染色体遗传图谱和物理图谱的比较分析表明，除了着丝粒区域的“冷点”外，沿着染色体臂还有3个遗传重组“热点”和2个“冷点”，这表明染色体的序列组成成分可能会影响其局部遗传重组频率。由于其具有植物转化能力，BIBAC/BAC图谱可为水稻基因组序列的功能分析以及将测序结果有效用于基因和QTL克隆及分子育种提供一个平台。
