Guan-Zhen Yu, Ying Chen, Can-Rong Ni, Guo-Dong Wang, Jian-Xin Qian, Jie-Jun Wang
Department of Oncology, Changzheng Hospital, Shanghai, China.
Int J Exp Pathol. 2007 Jun;88(3):175-83. doi: 10.1111/j.1365-2613.2006.00510.x.
Metastasis remains an incurable common complication in patients with gastric cancer. A variety of theories have been proposed to explain the inefficiency of the metastatic process. To compare protein expression of metastasis-related genes (nm23, KISS1, KAI1 and p53) between primary tumours and metastatic tumours may be useful in illustrating these theories.
Metastasis-related tissue microarrays (including normal tissues, primary tumours, nodal metastases and liver metastases) were constructed. The protein expression of nm23, KISS1, KAI1 and p53 in lymph node and liver metastases from advanced gastric cancer specimens was mainly examined by immunohistochemical staining in relation to primary tumours.
Immunohistochemical staining showed reduced protein expression of nm23, KISS1 and KAI1 in lymph node and liver metastases compared with primary tumours. Results for p53 were to the contrary.
Our investigations revealed a tendency of reduced protein expression of metastasis suppressor genes nm23, KISS1 and KAI1 in gastric cancer with the progress of metastasis. This means that the progression theory is an important determinant of metastatic efficiency.
转移仍然是胃癌患者一种无法治愈的常见并发症。人们提出了各种理论来解释转移过程的低效性。比较原发性肿瘤和转移性肿瘤之间转移相关基因(nm23、KISS1、KAI1和p53)的蛋白表达可能有助于阐明这些理论。
构建转移相关组织芯片(包括正常组织、原发性肿瘤、淋巴结转移灶和肝转移灶)。主要通过免疫组织化学染色检测晚期胃癌标本中淋巴结和肝转移灶中nm23、KISS1、KAI1和p53的蛋白表达,并与原发性肿瘤进行对比。
免疫组织化学染色显示,与原发性肿瘤相比,淋巴结和肝转移灶中nm23、KISS1和KAI1的蛋白表达降低。p53的结果则相反。
我们的研究表明,随着转移的进展,胃癌中转移抑制基因nm23、KISS1和KAI1的蛋白表达有降低的趋势。这意味着进展理论是转移效率的一个重要决定因素。