Ikeguchi Masahide, Yamaguchi Ken-ichi, Kaibara Nobuaki
Divisions of Operating Room, Faculty of Medicine, Tottori University, Yonago, Japan.
Clin Cancer Res. 2004 Feb 15;10(4):1379-83. doi: 10.1158/1078-0432.ccr-1519-02.
Lymph node metastasis is the most important predictor of prognosis in esophageal squamous cell carcinoma (ESCC). Recently, KiSS-1 was cloned as a human metastasis suppressor gene, and an orphan G-protein-coupled receptor (hOT7T175) was identified as the endogenous receptor of the KiSS-1 product. However, the clinical importance of KiSS-1 and hOT7T175 gene expression in ESCC remains unclear.
In this study, total RNA was extracted from tumors and noncancerous epithelia of 71 patients with ESCC who underwent surgical esophageal resection. The expression levels of KiSS-1, hOT7T175, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNAs were analyzed quantitatively by real-time reverse transcription-PCR and compared with the clinical findings.
The mean KiSS-1:GAPDH and hOT7T175:GAPDH ratios of the tumors were 1.2 and 0.3 and were at the same levels as those in the noncancerous epithelia. The loss of KiSS-1 and hOT7T175 gene expression was detected in 38% and 61% of tumors. Loss of KiSS-1 and/or hOT7T175 gene expression was not correlated with tumor size or degree of tumor invasion but was found to be a significant predictor of lymph node metastasis.
Loss of KiSS-1 or hOT7T175 gene expression may be an important biomarker for detection of lymph node metastasis in ESCC.
淋巴结转移是食管鳞状细胞癌（ESCC）预后的最重要预测指标。最近，KiSS-1被克隆为一种人类转移抑制基因，并且一种孤儿G蛋白偶联受体（hOT7T175）被鉴定为KiSS-1产物的内源性受体。然而，KiSS-1和hOT7T175基因表达在ESCC中的临床重要性仍不清楚。
在本研究中，从71例行食管手术切除的ESCC患者的肿瘤组织和非癌上皮组织中提取总RNA。通过实时逆转录PCR定量分析KiSS-1、hOT7T175和甘油醛-3-磷酸脱氢酶（GAPDH）mRNA的表达水平，并与临床结果进行比较。
肿瘤组织中KiSS-1:GAPDH和hOT7T175:GAPDH的平均比值分别为1.2和0.3，与非癌上皮组织中的水平相同。在38%的肿瘤中检测到KiSS-1基因表达缺失，在61%的肿瘤中检测到hOT7T175基因表达缺失。KiSS-1和/或hOT7T175基因表达缺失与肿瘤大小或肿瘤浸润程度无关，但被发现是淋巴结转移的重要预测指标。
KiSS-1或hOT7T175基因表达缺失可能是ESCC中检测淋巴结转移的重要生物标志物。
