使用体素化方法获得的小鼠脑切片的全基因组表达图谱。
A genome-scale map of expression for a mouse brain section obtained using voxelation.
作者信息
Chin Mark H, Geng Alex B, Khan Arshad H, Qian Wei-Jun, Petyuk Vladislav A, Boline Jyl, Levy Shawn, Toga Arthur W, Smith Richard D, Leahy Richard M, Smith Desmond J
机构信息
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
出版信息
Physiol Genomics. 2007 Aug 20;30(3):313-21. doi: 10.1152/physiolgenomics.00287.2006. Epub 2007 May 15.
Gene expression signatures in the mammalian brain hold the key to understanding neural development and neurological diseases. We have reconstructed two-dimensional images of gene expression for 20,000 genes in a coronal slice of the mouse brain at the level of the striatum by using microarrays in combination with voxelation at a resolution of 1 mm3. Good reliability of the microarray results were confirmed using multiple replicates, subsequent quantitative RT-PCR voxelation, mass spectrometry voxelation, and publicly available in situ hybridization data. Known and novel genes were identified with expression patterns localized to defined substructures within the brain. In addition, genes with unexpected patterns were identified, and cluster analysis identified a set of genes with a gradient of dorsal/ventral expression not restricted to known anatomical boundaries. The genome-scale maps of gene expression obtained using voxelation will be a valuable tool for the neuroscience community.
哺乳动物大脑中的基因表达特征是理解神经发育和神经疾病的关键。我们通过使用微阵列结合分辨率为1立方毫米的体素化技术，重建了小鼠大脑纹状体水平冠状切片中20000个基因的二维基因表达图像。通过多次重复实验、后续的定量逆转录聚合酶链反应体素化、质谱体素化以及公开可用的原位杂交数据，证实了微阵列结果具有良好的可靠性。我们识别出了已知基因和新基因，其表达模式定位于大脑内特定的子结构。此外，还发现了具有意外模式的基因，聚类分析确定了一组具有背侧/腹侧表达梯度且不限于已知解剖边界的基因。使用体素化获得的基因组规模基因表达图谱将成为神经科学界的宝贵工具。
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