Rodriguez-Tudela Juan L, Gomez-Lopez Alicia, Alastruey-Izquierdo Ana, Mellado Emilia, Bernal-Martinez Leticia, Cuenca-Estrella Manuel
Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda (Madrid), Spain.
Diagn Microbiol Infect Dis. 2007 Aug;58(4):435-40. doi: 10.1016/j.diagmicrobio.2007.03.001. Epub 2007 May 16.
The sequence polymorphisms of intergenic transcribed spacer and the antifungal susceptibility profile of 18 Trichosporon asahii isolates from Spain, Argentina, and Brazil together with 43 intergenic transcribed spacer 1 sequences deposited in the GenBank were analyzed. Six genotypes were detected instead of 5 genotypes described previously. Genotype 1 was the most common found comprising 57.3% of all strains, followed by genotype 3 (14.7%) and genotype 5 (13.1%). Spanish strains had members in all genotypes except 2, whereas South American isolates were grouped with genotypes 1, 3, and 6. Our results indicate that all genotypes are present in at least 2 countries suggesting a worldwide distribution. On the other hand, genotype 6 was not previously described but was only composed of 2 South American strains isolated from a subcutaneous abscess and skin. All isolates showed amphotericin B MICs>or=2 mg/L supporting the in vitro resistance of this species to this antifungal. Three isolates from South America showed high MICs to all antifungals analyzed. The true epidemiologic usefulness of classifying T. asahii in genotypes should be ascertaining analyzing a high number of isolates from many countries.
分析了来自西班牙、阿根廷和巴西的18株阿萨希毛孢子菌分离株的基因间隔转录间隔区序列多态性和抗真菌药敏谱，以及GenBank中保存的43个基因间隔转录间隔区1序列。检测到6种基因型，而非先前描述的5种基因型。基因型1是最常见的，占所有菌株的57.3%，其次是基因型3（14.7%）和基因型5（13.1%）。西班牙菌株除基因型2外，在所有基因型中均有成员，而南美分离株则归为基因型1、3和6。我们的结果表明，所有基因型至少在2个国家存在，提示其分布于全球。另一方面，基因型6以前未被描述，仅由从皮下脓肿和皮肤分离的2株南美菌株组成。所有分离株的两性霉素B MIC≥2mg/L，支持该菌种对这种抗真菌药的体外耐药性。来自南美的3株分离株对所有分析的抗真菌药均显示出高MIC。通过分析来自许多国家的大量分离株来确定将阿萨希毛孢子菌按基因型分类的真正流行病学用途。
