Premamalini Thayanidhi, Rajyoganandh Vijayaraman, Vijayakumar Ramaraj, Veena Hemanth, Kindo Anupma Jyoti, Marak Rungmei Sk
Department of Microbiology, Sri Ramachandra Medical College & Research Institute, SRIHER, Porur, Chennai, Tamil Nadu, India.
Department of Microbiology, Vels Institute of Science, Technology and Advanced Studies, Vels Institute, Chennai, Tamil Nadu, India.
J Lab Physicians. 2021 Sep;13(3):245-251. doi: 10.1055/s-0041-1731111. Epub 2021 Jul 2.
The aim of this study was to identify and isolate ( ) from clinical samples and to assess the genetic relatedness of the most frequently isolated strains of using random amplification of polymorphic DNA (RAPD) primers GAC-1 and M13. All the clinical samples that grew species, identified and confirmed by polymerase chain reaction (PCR) using genus-specific primers, were considered for the study. Confirmation of the species was carried out by specific PCR. Fingerprinting of the most frequently isolated isolates was carried out by RAPD using random primers GAC-1 and M13. Among the 72 clinical isolates of sp. confirmed by -specific PCR, 65 were found to be as identified by specific PCR. Fingerprinting of the 65 isolates confirmed as using GAC-1 RAPD primer yielded 11 different patterns, whereas that of M13 primer produced only 5 patterns. The pattern I was found to be the most predominant type (29.2%) followed by pattern III (16.9%) by GAC-1 primer. This study being the first of its kind in India on strain typing of isolates by adopting RAPD analysis throws light on genetic diversity among the isolates from clinical samples. Fingerprinting by RAPD primer GAC-1 identified more heterogeneity among the isolates than M13.
本研究的目的是从临床样本中鉴定并分离出（ ），并使用多态性DNA随机扩增（RAPD）引物GAC - 1和M13评估最常分离菌株的遗传相关性。所有通过使用属特异性引物的聚合酶链反应（PCR）鉴定并确认生长有（ ）种的临床样本均纳入本研究。通过（ ）特异性PCR对菌种进行确认。使用随机引物GAC - 1和M13通过RAPD对最常分离的（ ）分离株进行指纹图谱分析。在通过（ ）特异性PCR确认的72株（ ）临床分离株中，经（ ）特异性PCR鉴定发现65株为（ ）。使用GAC - 1 RAPD引物对确认为（ ）的65株分离株进行指纹图谱分析产生了11种不同模式，而M13引物产生了仅5种模式。发现模式I是最主要的类型（29.2%），其次是GAC - 1引物的模式III（16.9%）。本研究是印度首例采用RAPD分析对（ ）分离株进行菌株分型的研究，揭示了临床样本中（ ）分离株之间的遗传多样性。与M13相比，RAPD引物GAC - 1在（ ）分离株中鉴定出更多的异质性。
