Strain Typing of Clinical Isolates by Random Amplification of Polymorphic DNA (RAPD) Analysis.

The aim of this study was to identify and isolate ( ) from clinical samples and to assess the genetic relatedness of the most frequently isolated strains of using random amplification of polymorphic DNA (RAPD) primers GAC-1 and M13. All the clinical samples that grew species, identified and confirmed by polymerase chain reaction (PCR) using genus-specific primers, were considered for the study. Confirmation of the species was carried out by specific PCR. Fingerprinting of the most frequently isolated isolates was carried out by RAPD using random primers GAC-1 and M13. Among the 72 clinical isolates of sp. confirmed by -specific PCR, 65 were found to be as identified by specific PCR. Fingerprinting of the 65 isolates confirmed as using GAC-1 RAPD primer yielded 11 different patterns, whereas that of M13 primer produced only 5 patterns. The pattern I was found to be the most predominant type (29.2%) followed by pattern III (16.9%) by GAC-1 primer. This study being the first of its kind in India on strain typing of isolates by adopting RAPD analysis throws light on genetic diversity among the isolates from clinical samples. Fingerprinting by RAPD primer GAC-1 identified more heterogeneity among the isolates than M13.