Walencik Jacek, Witeska Małgorzata
Department of Animal Physiology, University of Podlasie, Prusa 12, 08110 Siedlce, Poland.
Comp Biochem Physiol C Toxicol Pharmacol. 2007 Sep;146(3):331-5. doi: 10.1016/j.cbpc.2007.04.004. Epub 2007 Apr 19.
Hematological parameters (Ht, Hb, RBC, WBC, PLT), erythrocyte size, and osmotic fragility, differential leukocyte count, ROS production in common carp blood collected on three anticoagulants: heparin (10 IU/mL, Na2EDTA (0.1, 0.5, and 1 mg/mL), and sodium citrate (0.3 mg/mL) were compared. Na2EDTA caused partial blood hemolysis in Ht tubes which made Ht measurement impossible, and resulted in high variability of the results. Both, citrate and Na2EDTA increased sensitivity of red blood cells to hemolysis. Na2EDTA also induced erythrocyte anisocytosis and anisonucleosis. Na2EDTA significantly increased ROS production but no effect of anticoagulants on WBC, PLT or differential leukocyte count was observed. The obtained results show that Na2EDTA should not be used for evaluation of red blood cell parameters and erythrocyte morphology, and for ROS production measurement in common carp. Heparin proved to be the most appropriate anticoagulant to use for this species, although Na2EDTA and sodium citrate may be used for WBC and leukocyte differential count evaluations.
比较了三种抗凝剂（肝素（10 IU/mL）、乙二胺四乙酸二钠（0.1、0.5和1 mg/mL）和柠檬酸钠（0.3 mg/mL））采集的鲤鱼血液中的血液学参数（红细胞压积、血红蛋白、红细胞、白细胞、血小板）、红细胞大小、渗透脆性、白细胞分类计数以及活性氧生成情况。乙二胺四乙酸二钠在红细胞压积管中导致部分血液溶血，使得无法进行红细胞压积测量，并导致结果的高变异性。柠檬酸钠和乙二胺四乙酸二钠均增加了红细胞对溶血的敏感性。乙二胺四乙酸二钠还诱导了红细胞大小不均一性和核大小不均一性。乙二胺四乙酸二钠显著增加了活性氧生成，但未观察到抗凝剂对白细胞、血小板或白细胞分类计数有影响。所得结果表明，乙二胺四乙酸二钠不应用于评估鲤鱼的红细胞参数和红细胞形态以及活性氧生成测量。肝素被证明是用于该物种的最合适的抗凝剂，尽管乙二胺四乙酸二钠和柠檬酸钠可用于白细胞和白细胞分类计数评估。
