Mokhtari Tahmineh, Hussein Osman Hosam-Eldin, El-Meghawry El-Kenawy Ayman, Dashti Nasrin
Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, College of Medicine, Taif University, Al-Azhar University, Saudi Arabia.
J Tradit Complement Med. 2019 Aug 28;10(5):487-495. doi: 10.1016/j.jtcme.2019.08.005. eCollection 2020 Sep.
Ethephon (EP) is the most famous plant growth regulator with different adverse effects on kidney function. Virgin Olive Oil (VOO) is considered as a natural source of antioxidant with beneficial effects. Thus, this study was conducted to investigate the effects of VOO on nephrotoxicity induced by EP in rats.
In this study, 80 male rats (weighing 200-250 g) were divided into four groups including : control group received normal saline as vehicle, received VOO, received EP (150 mg/kg/day) for 2 months, received EP (150 mg/kg/day for 2 months, after 2-month pretreatment with VOO. VOO (2 mL/kg/day) and vehicle were administered by gastric gavage for 2 months. At the end, the animals were sacrificed, and their blood and kidneys were used for examinations. Isolated kidneys were used for histopathological and oxidative stress studies.
Significant increases were recorded in blood (neutrophils, monocytes) and urinary parameters as well as malondialdehyde (MDA) content in the group III compared to groups II and I (P˂0.05). Antioxidant enzymes significantly declined and histopathological alterations increased in the group III. In the group IV, significant decreases were recorded in blood and urinary parameters, MDA, and histopathological alterations and a significant increase were found in antioxidant enzymes compared to group III (P˂0.05).
Findings of the present study demonstrated protective effects of VOO in prevention of kidneys against EP -induced toxicity in albino rats.
乙烯利（EP）是最著名的植物生长调节剂，对肾功能有不同的不良影响。初榨橄榄油（VOO）被认为是一种具有有益作用的天然抗氧化剂来源。因此，本研究旨在探讨VOO对EP诱导的大鼠肾毒性的影响。
在本研究中，80只雄性大鼠（体重200 - 250克）被分为四组，包括：对照组接受生理盐水作为赋形剂；组接受VOO；组接受EP（150毫克/千克/天），持续2个月；组在经VOO预处理2个月后，接受EP（150毫克/千克/天，持续2个月）。VOO（2毫升/千克/天）和赋形剂通过灌胃给药2个月。最后，处死动物，取其血液和肾脏进行检查。分离的肾脏用于组织病理学和氧化应激研究。
与组II和组I相比，组III的血液（中性粒细胞、单核细胞）和尿液参数以及丙二醛（MDA）含量显著增加（P˂0.05）。组III的抗氧化酶显著下降，组织病理学改变增加。与组III相比，组IV的血液和尿液参数、MDA以及组织病理学改变显著降低，抗氧化酶显著增加（P˂0.05）。
本研究结果表明，VOO对白化病大鼠肾脏具有保护作用，可预防EP诱导的毒性。
