[应用定量实时聚合酶链反应方法检测羊水中的弓形虫]
[Detection of Toxoplasma gondii in amniotic fluid using quantitative real-time PCR method].
作者信息
Nagy Bálint, Lázár Levente, Nagy Gyula, Bán Zoltán, Papp Zoltán
机构信息
Semmelweis Egyetem, Altalános Orvostudományi Kar, I. Szülészeti és Nogyógyászati Klinika, Budapest.
出版信息
Orv Hetil. 2007 May 20;148(20):935-8. doi: 10.1556/OH.2007.27971.
INTRODUCTION
The infection caused by parasite Toxoplasma gondii is often asymptomatic or a mild clinical disease. Congenital toxoplasmosis is the result of transplacental transmission of Toxoplasma gondii from an acute infected mother. Toxoplasmosis can cause several fetal symptoms. Early diagnosis of the infection can enhance the success of the medical treatment. Congenital toxoplasmosis can be detected by serological or PCR amplification methods.
AIMS
The authors decided to develop a quantitative real-time PCR technique for detection of T. gondii in amniotic fluid samples.
METHODS
DNA was isolated using silica adsorption method. Quantitative real-time PCR method was used to detect T. gondii infection in the samples.
RESULTS
From the studied 74 samples in 6 cases T. gondii was detected.
CONCLUSION
The introduced quantitative real-time PCR method is a fast and sensitive PCR based method and makes possible the quantification of the protozoa number in the sample.
引言
由寄生虫弓形虫引起的感染通常无症状或为轻度临床疾病。先天性弓形虫病是急性感染的母亲经胎盘传播弓形虫的结果。弓形虫病可导致多种胎儿症状。感染的早期诊断可提高治疗成功率。先天性弓形虫病可通过血清学或聚合酶链反应（PCR）扩增方法检测。
目的
作者决定开发一种定量实时PCR技术，用于检测羊水样本中的弓形虫。
方法
采用硅胶吸附法分离DNA。使用定量实时PCR方法检测样本中的弓形虫感染。
结果
在所研究的74个样本中，检测到6例弓形虫。
结论
所引入的定量实时PCR方法是一种基于PCR的快速且灵敏的方法，能够对样本中的原生动物数量进行定量。
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