Dasti Javid Iqbal, Groß Uwe, Pohl Sven, Lugert Raimond, Weig Michael, Schmidt-Ott Ruprecht
Institute of Medical Microbiology, University of Göttingen, D-37075 Göttingen, Germany.
J Med Microbiol. 2007 Jun;56(Pt 6):833-837. doi: 10.1099/jmm.0.47103-0.
The prevalence of tetracycline resistance, tetracycline MICs and tet(O) gene localization were investigated in 83 Campylobacter isolates from patients suffering from acute gastroenteritis in Germany. Combined biochemical and molecular markers identified 74 isolates (89 %) as Campylobacter jejuni, including seven atypical isolates that failed to hydrolyse hippurate, and nine isolates (11 %) as Campylobacter coli. Tetracycline resistance was detected in six out of nine Campylobacter coli isolates (67 %) and 13 out of 74 C. jejuni isolates (18 %). Low-level tetracycline resistance was observed for C. coli (MIC 16 microg ml(-1) for all strains), whereas C. jejuni showed high-level resistance (MIC >256 microg ml(-1) for all strains). Both low- and high-level tetracycline resistance was associated with the presence of the tet(O) gene. In C. jejuni, tet(O) was plasmid-encoded in 54 % of tetracycline-resistant isolates, whereas in C. coli, tet(O) appeared to be located on the chromosome.
对德国83株来自急性肠胃炎患者的弯曲杆菌分离株进行了四环素耐药性、四环素最低抑菌浓度（MIC）及tet(O)基因定位的研究。联合生化和分子标记鉴定出74株（89%）为空肠弯曲菌，其中包括7株不能水解马尿酸盐的非典型分离株，9株（11%）为大肠弯曲菌。在9株大肠弯曲菌分离株中有6株（67%）检测到四环素耐药，74株空肠弯曲菌分离株中有13株（18%）检测到四环素耐药。大肠弯曲菌呈现低水平四环素耐药（所有菌株的MIC为16μg/ml），而空肠弯曲菌呈现高水平耐药（所有菌株的MIC>256μg/ml）。低水平和高水平四环素耐药均与tet(O)基因的存在有关。在空肠弯曲菌中，54%的四环素耐药分离株中tet(O)是由质粒编码的，而在大肠弯曲菌中，tet(O)似乎位于染色体上。
