Lee C Y, Tai C L, Lin S C, Chen Y T
Department of Agricultural Chemistry, National Taiwan University, Taipei.
Int J Food Microbiol. 1994 Dec;24(1-2):161-70. doi: 10.1016/0168-1605(94)90115-5.
Twenty whole market chickens, purchased from 10 different stores in the Taipei Metropolitan area, were examined for the presence of Campylobacter jejuni and Campylobacter coli. The microorganisms were recovered from 95% of the chickens. A survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. One hundred and sixty-seven chicken isolates and the 41 clinical isolates of Campylobacter jejuni were examined for the occurrence of plasmid DNA in association with tetracycline resistance. A high plasmid occurrence rate of 91% and 44% was observed for C. jejuni from chickens and clinical isolates, respectively. Plasmids ranged in size from 16 to 208 Kb. A 61 Kb plasmid and a 50 Kb plasmid were common to the chicken isolates and clinical isolates, respectively. All chicken isolates and 78% of clinical isolates were tetracycline-resistant. The high rate of tetracycline resistance in chicken isolates probably related to use of tetracycline as a growth promoter for poultry. A tetO DNA Probe, highly specific for the detection of tetracycline resistance in C. jejuni and C. coli, was used to find the location of tetracycline resistance. Of 157 chicken isolates, 98% of isolates were positive with the tetO probe, 87% (137/157) on plasmids and 11% (17/157) on the chromosome; only three isolates did not hybridize with the tetO probe. Of 32 clinical isolates, 88% isolates hybridized with the tetO probe, 47% (15/32) on plasmids and 41% (13/32) on the chromosome; four isolates did not hybridize with the tetO probe.
从台北都会区10家不同商店购买了20只市售整鸡，检测空肠弯曲菌和结肠弯曲菌的存在情况。在95%的鸡中检出了这些微生物。对鸡胸、大腿和尾部等不同部位的调查显示，所有这些部位的污染情况同样普遍。对167株鸡源弯曲菌分离株和41株空肠弯曲菌临床分离株进行了质粒DNA与四环素耐药性相关性的检测。鸡源空肠弯曲菌和临床分离株的质粒出现率分别高达91%和44%。质粒大小在16至208 kb之间。鸡源分离株和临床分离株分别常见61 kb质粒和50 kb质粒。所有鸡源分离株和78%的临床分离株对四环素耐药。鸡源分离株中四环素耐药率高可能与将四环素用作家禽生长促进剂有关。使用一种对空肠弯曲菌和结肠弯曲菌中四环素耐药性检测具有高度特异性的tetO DNA探针来确定四环素耐药性的位置。在157株鸡源分离株中，98%的分离株tetO探针检测呈阳性，87%（137/157）位于质粒上，11%（17/157）位于染色体上；只有3株分离株未与tetO探针杂交。在32株临床分离株中，88%的分离株与tetO探针杂交，47%（15/32）位于质粒上，41%（13/32）位于染色体上；4株分离株未与tetO探针杂交。
