Kapranov Philipp, Cheng Jill, Dike Sujit, Nix David A, Duttagupta Radharani, Willingham Aarron T, Stadler Peter F, Hertel Jana, Hackermüller Jörg, Hofacker Ivo L, Bell Ian, Cheung Evelyn, Drenkow Jorg, Dumais Erica, Patel Sandeep, Helt Gregg, Ganesh Madhavan, Ghosh Srinka, Piccolboni Antonio, Sementchenko Victor, Tammana Hari, Gingeras Thomas R
Affymetrix Laboratory, Affymetrix, Inc., 3420 Central Expressway, Santa Clara, CA, 95051, USA.
Science. 2007 Jun 8;316(5830):1484-8. doi: 10.1126/science.1138341. Epub 2007 May 17.
Significant fractions of eukaryotic genomes give rise to RNA, much of which is unannotated and has reduced protein-coding potential. The genomic origins and the associations of human nuclear and cytosolic polyadenylated RNAs longer than 200 nucleotides (nt) and whole-cell RNAs less than 200 nt were investigated in this genome-wide study. Subcellular addresses for nucleotides present in detected RNAs were assigned, and their potential processing into short RNAs was investigated. Taken together, these observations suggest a novel role for some unannotated RNAs as primary transcripts for the production of short RNAs. Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes. These data support a highly interleaved organization of the human transcriptome.
相当一部分真核生物基因组会产生RNA，其中许多RNA未被注释且蛋白质编码潜力降低。在这项全基因组研究中，对长度超过200个核苷酸（nt）的人类细胞核和细胞质多聚腺苷酸化RNA以及长度小于200 nt的全细胞RNA的基因组起源和关联进行了研究。为检测到的RNA中存在的核苷酸分配了亚细胞定位，并研究了它们加工成短RNA的潜力。综合这些观察结果表明，一些未注释的RNA作为产生短RNA的初级转录本具有新的作用。已鉴定出三类具有潜在功能的RNA，其中两类在染色体上保守且与蛋白质编码基因的表达状态相关。这些数据支持人类转录组的高度交错组织。
