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本文引用的文献

1
The complexity of mitochondrial tRNA import.线粒体转运RNA导入的复杂性
RNA Biol. 2004 Jul;1(2):84-8. doi: 10.4161/rna.1.2.1180. Epub 2004 Jul 16.
2
The voltage-dependent anion channel, a major component of the tRNA import machinery in plant mitochondria.电压依赖性阴离子通道，植物线粒体中tRNA导入机制的主要组成部分。
Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18362-7. doi: 10.1073/pnas.0606449103. Epub 2006 Nov 14.
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Functional delivery of a cytosolic tRNA into mutant mitochondria of human cells.将胞质转运RNA功能性递送至人类细胞的突变线粒体中。
Science. 2006 Oct 20;314(5798):471-4. doi: 10.1126/science.1129754.
4
An RNA-binding respiratory component mediates import of type II tRNAs into Leishmania mitochondria.一种RNA结合呼吸组分介导II型tRNA导入利什曼原虫线粒体。
J Biol Chem. 2006 Sep 1;281(35):25270-7. doi: 10.1074/jbc.M604126200. Epub 2006 Jul 6.
5
A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae.在酿酒酵母中，一种糖酵解酶烯醇化酶作为tRNA靶向线粒体的辅助因子被招募。
Genes Dev. 2006 Jun 15;20(12):1609-20. doi: 10.1101/gad.385706. Epub 2006 May 31.
6
A bifunctional tRNA import receptor from Leishmania mitochondria.来自利什曼原虫线粒体的双功能tRNA导入受体。
Proc Natl Acad Sci U S A. 2006 May 30;103(22):8354-9. doi: 10.1073/pnas.0510869103. Epub 2006 May 19.
7
Correction of translational defects in patient-derived mutant mitochondria by complex-mediated import of a cytoplasmic tRNA.通过细胞质tRNA的复合物介导导入纠正患者来源的突变线粒体中的翻译缺陷。
J Biol Chem. 2005 Feb 18;280(7):5141-4. doi: 10.1074/jbc.C400572200. Epub 2004 Dec 24.
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Moonlighting proteins: old proteins learning new tricks.兼职蛋白：老蛋白学新招。
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"Ping-pong" interactions between mitochondrial tRNA import receptors within a multiprotein complex.多蛋白复合物中线粒体tRNA导入受体之间的“乒乓”相互作用。
Mol Cell Biol. 2003 Aug;23(15):5217-24. doi: 10.1128/MCB.23.15.5217-5224.2003.
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转运RNA导入动质体线粒体的必要和充分因素。

Necessary and sufficient factors for the import of transfer RNA into the kinetoplast mitochondrion.

作者信息

Mukherjee Saikat, Basu Sudarshana, Home Pratik, Dhar Gunjan, Adhya Samit

机构信息

Genetic Engineering Laboratory, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700032, India.

出版信息

EMBO Rep. 2007 Jun;8(6):589-95. doi: 10.1038/sj.embor.7400979. Epub 2007 May 18.

DOI:10.1038/sj.embor.7400979
PMID:17510656
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2002530/
Abstract

The mechanism of active transport of transfer RNA (tRNA) across membranes is largely unknown. Factors mediating the import of tRNA into the kinetoplast mitochondrion of the protozoon Leishmania tropica are organized into a multiprotein RNA import complex (RIC) at the inner membrane. Here, we present the complete characterization of the identities and functions of the subunits of this complex. The complex contains three mitochondrion- and eight nuclear-encoded subunits; six of the latter are necessary and sufficient for import. Antisense-mediated knockdown of essential subunits resulted in the depletion of mitochondrial tRNAs and inhibition of organellar translation. Functional complexes were reconstituted with recombinant subunits expressed in Escherichia coli. Several essential RIC subunits are identical to specific subunits of respiratory complexes. These findings provide new information on the evolution of tRNA import and the foundation for detailed structural and mechanistic studies.

摘要

转运核糖核酸（tRNA）跨膜主动运输的机制在很大程度上尚不清楚。介导tRNA导入热带利什曼原虫动质体线粒体的因子在内膜处组装成一个多蛋白RNA导入复合物（RIC）。在此，我们展示了该复合物亚基的身份和功能的完整特征。该复合物包含三个线粒体编码亚基和八个核编码亚基；后者中的六个对于导入是必需且足够的。通过反义介导敲低必需亚基导致线粒体tRNA耗竭并抑制细胞器翻译。用在大肠杆菌中表达的重组亚基重建了功能复合物。几个必需的RIC亚基与呼吸复合物的特定亚基相同。这些发现为tRNA导入的进化提供了新信息，并为详细的结构和机制研究奠定了基础。