Potts Ryan J, Meckley Daniel R, Shreve W Keith, Pence Deborah H, Ayres Paul H, Doolittle David, Swauger James E, Sagartz John W
Scientific and Regulatory Affairs, R. J. Reynolds Tobacco Company, Winston-Salem, North Carolina 27102, USA.
Inhal Toxicol. 2007 Jun;19(8):701-24. doi: 10.1080/08958370701353007.
A subchronic, nose-only inhalation study was conducted to compare the effects of mainstream smoke from a reference cigarette containing conventional reconstituted tobacco sheet at 30% of the finished blend to mainstream smoke from cigarettes containing 10% or 15% cast sheet (a specific type of reconstituted tobacco sheet) substituted for part of the conventional reconstituted tobacco. Male and female Sprague-Dawley rats were exposed for 1 h/day, 5 d/wk, for 13 wk to mainstream smoke at 0, 0.06, 0.20, or 0.80 mg wet total particulate matter per liter of air. Clinical signs, body and organ weights, clinical chemistry, hematology, carboxyhemoglobin (COHb), serum nicotine, plethysmography, gross pathology, and histopathology were determined. Exposure to cigarette smoke induced a number of changes in respiratory physiology, histopathology, and serum nicotine and COHb levels when compared to sham animals. When corresponding dose groups of reference and cast sheet mainstream smokes were compared, no biological differences were noted. At the end of the exposure period, subsets of rats from each group were maintained without smoke exposures for an additional 13 wk (recovery period). At the end of the recovery period, there were no statistically significant differences in histopathological findings observed between the reference and either cast sheet cigarette. Substitution of 10% or 15% cast sheet tobacco for conventional reconstituted tobacco sheet does not alter the inhalation toxicology of the mainstream smoke when compared to mainstream smoke from a reference cigarette containing conventional reconstituted tobacco sheet.
进行了一项亚慢性、仅经鼻吸入研究，以比较含有30%成品混合比例的传统重组烟草薄片的参比卷烟主流烟气与含有10%或15%铸型薄片（一种特定类型的重组烟草薄片）替代部分传统重组烟草的卷烟主流烟气的影响。将雄性和雌性斯普拉格-道利大鼠每天暴露1小时，每周5天，持续13周，暴露于每升空气中含有0、0.06、0.20或0.80毫克湿总颗粒物的主流烟气中。测定了临床体征、身体和器官重量、临床化学、血液学、碳氧血红蛋白（COHb）、血清尼古丁、体积描记法、大体病理学和组织病理学。与假手术动物相比，暴露于香烟烟雾会引起呼吸生理学、组织病理学以及血清尼古丁和COHb水平的一些变化。当比较参比卷烟和铸型薄片卷烟主流烟气的相应剂量组时，未发现生物学差异。在暴露期结束时，每组的部分大鼠在无烟雾暴露的情况下再维持13周（恢复期）。在恢复期结束时，参比卷烟和铸型薄片卷烟之间在组织病理学检查结果上没有统计学上的显著差异。与含有传统重组烟草薄片的参比卷烟主流烟气相比，用10%或1
