Potts Ryan J, Bombick Betsy R, Meckley Daniel R, Ayres Paul H, Pence Deborah H
Research and Development, R. J. Reynolds Tobacco Company, Bowman Gray Technical Center, P. O. Box 1487, Winston-Salem, NC 27102, USA.
Exp Toxicol Pathol. 2010 Mar;62(2):117-26. doi: 10.1016/j.etp.2009.02.121. Epub 2009 Apr 8.
A tiered testing strategy based on a comparative chemical and biological testing program has been developed to evaluate the potential of tobacco processes, ingredients, or other technological developments to change the biological activity that results from burning tobacco. Cast sheet tobacco is a specific type of reconstituted tobacco sheet that can be used in the manufacture of cigarettes. The comparative chemical and biological testing program was used to compare the mainstream smoke and cigarette smoke condensate (CSC) from a Reference cigarette that did not contain cast sheet to that collected from Test cigarettes containing cast sheet at a final blend level of either 10% or 15%. Testing included mainstream cigarette smoke chemistry studies, in vitro studies (Ames assay, sister chromatid exchange assay, and neutral red cytotoxicity assay), and in vivo toxicology studies (13-week rat nose-only inhalation assay and 30-week mouse dermal tumor promotion assay). Certain statistically significant differences were observed in the chemical and biological studies when the Reference cigarette was compared to each of the Test cigarettes. However, when viewed collectively, the chemical and biological studies demonstrated that inclusion of cast sheet up to 15% in the final blend did not increase the inherent biological activity of mainstream cigarette smoke or CSC.
已制定了一种基于比较化学和生物学测试计划的分级测试策略，以评估烟草加工过程、成分或其他技术发展改变燃烧烟草所产生生物活性的可能性。铸型薄片烟草是一种特定类型的再造烟草薄片，可用于制造香烟。比较化学和生物学测试计划用于比较不含铸型薄片的参比卷烟与最终混合比例为10%或15%的含铸型薄片测试卷烟的主流烟气和卷烟烟气冷凝物（CSC）。测试包括主流卷烟烟气化学研究、体外研究（艾姆斯试验、姐妹染色单体交换试验和中性红细胞毒性试验）以及体内毒理学研究（13周大鼠仅经鼻吸入试验和30周小鼠皮肤肿瘤促进试验）。当将参比卷烟与各测试卷烟进行比较时，在化学和生物学研究中观察到了某些具有统计学意义的差异。然而，综合来看，化学和生物学研究表明，在最终混合物中加入高达15%的铸型薄片不会增加主流卷烟烟气或CSC的固有生物活性。
