Te Dorsthorst D T A, Zwaaftink R B M Groot, Rijs A J M M, Meletiadis J, Verweij P E
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital Nijmegen, Nijmegen, The Netherlands.
Med Mycol. 2007 Jun;45(4):351-5. doi: 10.1080/13693780701216485.
The in vitro susceptibility of 45 Aspergillus fumigatus, Aspergillus flavus and Aspergillus terreus isolates against caspofungin (CAS) was assessed by the CLSI reference method with spectrophotometric reading and by a colorimetric method that employed the dye MTT. Perfect agreement was found between the minimal effective concentrations (MECs) and the MIC-2 values (50% growth reduction) obtained by the MTT method. The agreement found between the MICs obtained by the CLSI and the MTT method was dependent on the MIC endpoint used, the antifungal agent tested, and the species investigated.
采用CLSI参考方法通过分光光度法读数以及采用染料MTT的比色法，评估了45株烟曲霉、黄曲霉和土曲霉分离株对卡泊芬净（CAS）的体外敏感性。MTT法获得的最小有效浓度（MEC）与MIC-2值（生长降低50%）之间发现完全一致。CLSI法与MTT法获得的MIC之间的一致性取决于所使用的MIC终点、所测试的抗真菌剂以及所研究的菌种。
