Zhang Wan-Dong, Lu Cheng, Zhao Hong-Yan, Chen Shi-Lin, Yang Da-Jian, Lu Ai-Ping
Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):414-7.
To observe the effects of combination therapy with glycyrrhizin (GL) and triptolide (TP) on collagen-induced arthritis (CIA) rats.
Sixty male SD rats were randomly divided into 6 groups: the model group, the TP group, the GL group, and combination 1, 2, 3 groups. The models were induced by collagen type II. The arthritis index (AI) and the edema rate were detected as curative effect, and the level of antibodies to collagen, TNF-alpha and IL-10 were measured by ELISA.
The combination therapy with GL and TP significantly reduced the paw edema and arthritis index of CIA rats (P <0. 01 ), and the combination therapy can increase the level of IL-10, while decrease the level of TNF-alpha, and the level of antibodies to collagen decreased too (P <0.05, P <0.01).
Combine 26.78 mg x kg(-1) GL with 13.40 microg x kg(-1) TP can significantly inhibited the CIA, and the effect equal to the dosage of 17. 86 microg x kg(-1) TP. It supports the possible of GL in combination with TP to reduce the dose and side effects related to TP.
观察甘草酸（GL）与雷公藤甲素（TP）联合治疗对胶原诱导性关节炎（CIA）大鼠的影响。
将60只雄性SD大鼠随机分为6组：模型组、TP组、GL组以及联合1、2、3组。采用Ⅱ型胶原诱导模型。检测关节炎指数（AI）和水肿率作为疗效指标，并用酶联免疫吸附测定法（ELISA）检测抗胶原抗体、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）水平。
GL与TP联合治疗显著降低了CIA大鼠的爪部水肿和关节炎指数（P<0.01），联合治疗可提高IL-10水平，同时降低TNF-α水平，抗胶原抗体水平也降低（P<0.05，P<0.01）。
26.78mg·kg⁻¹的GL与13.40μg·kg⁻¹的TP联合使用可显著抑制CIA，其效果等同于17.86μg·kg⁻¹的TP剂量。这支持了GL与TP联合使用可能降低TP相关剂量和副作用的可能性。
