Polpanich Duangporn, Tangboriboonrat Pramuan, Elaissari Abdelhamid, Udomsangpetch Rachanee
Department of Chemistry, Faculty of Science, Mahidol University, Rama VI Rd, Phyathai, Bangkok 10400, Thailand.
Anal Chem. 2007 Jun 15;79(12):4690-5. doi: 10.1021/ac070502w. Epub 2007 May 19.
A rapid test for malaria diagnosis based on specific agglutination of sensitive polystyrene particles containing carboxylic acid with antigen or antibody molecules in the presence of their corresponding antibody or antigen in human plasma has been achieved. The particle-malaria antigen conjugate (PAgC), particle-monoclonal IgG antibody to Plasmodium falciparum heat shock protein 70 conjugate (PmAbC), and particle-polyclonal IgG antibody to P. falciparum malaria conjugate (PpAbC) were prepared via adsorption process. The higher affinity of the malaria antigen adsorption onto particles was observed compared to that of the antibodies. Immunoagglutination of sensitive latex particles was monitored by measuring the change in turbidity, and the aggregate's formation was clearly observed under optical microscope. The efficacy in malaria diagnosis of the conjugated particles evaluated at an outpatient malaria clinic (Mae Sod, Thailand) indicated a success detection of antibody or antigen. Sensitivity of PAgC, PmAbC, and PpAbC for P. falciparum was 84%, 90%, and 90%, respectively, while specificity for malaria disease was 70% for PAgC and 80% for PmAbC and PpAbC. The rapid agglutination-based latex particles assay developed herein showed a great potential for diagnosis of malaria infection.
已实现一种基于敏感的含羧酸聚苯乙烯颗粒在人血浆中与其相应抗体或抗原存在时与抗原或抗体分子特异性凝集的疟疾诊断快速检测方法。通过吸附过程制备了颗粒 - 疟疾抗原缀合物（PAgC）、恶性疟原虫热休克蛋白70单克隆IgG抗体颗粒缀合物（PmAbC）和恶性疟原虫疟疾多克隆IgG抗体颗粒缀合物（PpAbC）。与抗体相比，观察到疟疾抗原在颗粒上的吸附亲和力更高。通过测量浊度变化监测敏感乳胶颗粒的免疫凝集，并在光学显微镜下清楚地观察到聚集体的形成。在泰国湄索的一家门诊疟疾诊所评估的缀合颗粒在疟疾诊断中的功效表明成功检测到了抗体或抗原。PAgC、PmAbC和PpAbC对恶性疟原虫的敏感性分别为84%、90%和90%，而对疟疾疾病的特异性，PAgC为70%，PmAbC和PpAbC为80%。本文开发的基于快速凝集的乳胶颗粒检测方法在疟疾感染诊断方面显示出巨大潜力。
