Kutateladze Tamara, Karchkhadze Kakha, Bitskinashvili Kakha, Vishnepolsky Boris, Ninidze Tata, Mikeladze David, Datukishvili Nelly
Ivane Beritashvili Center of Experimental Biomedicine, 14 Gotua Str., Tbilisi 0160, Georgia.
School of Natural Sciences and Medicine, Ilia State University, 3/5 Kakutsa Cholokashvili Ave., Tbilisi 0162, Georgia.
Foods. 2024 Nov 24;13(23):3760. doi: 10.3390/foods13233760.
Reliable detection of sunflower () in edible and used cooking oil (UCO) is crucial for the sustainable production of food and biodiesel. In this study, a variety of sunflower oils (crude, cold pressed, extra virgin, refined, and UCO) were examined using different methods of DNA extraction and PCR amplification to develop an efficient technology for the identification of sunflower in oils. DNA extraction kits such as NucleoSpin Food, DNeasy mericon Food, and Olive Oil DNA Isolation as well as modified CTAB method were found to be able to isolate amplifiable genomic DNA from highly processed oils. Novel uniplex, double, and nested PCR systems targeting the sunflower-specific gene were developed for efficient identification of sunflower. New sunflower DNA markers were revealed by uniplex PCRs. The combination of modified CTAB and nested PCR was demonstrated as a reliable, rapid, and cost-effective technology for detecting traces of sunflower in 700 μL of highly processed oil, including refined and used cooking oil. The study will contribute to both the food industry and the energy sector as developed methods can be used for oil authenticity testing in food and biodiesel production.
可靠检测食用和使用过的烹饪油（UCO）中的向日葵（ ）对于食品和生物柴油的可持续生产至关重要。在本研究中，使用不同的DNA提取和PCR扩增方法对多种向日葵油（粗制、冷榨、特级初榨、精炼和UCO）进行了检测，以开发一种用于鉴定油中向日葵的高效技术。发现诸如NucleoSpin Food、DNeasy mericon Food和橄榄油DNA分离试剂盒以及改良CTAB法等DNA提取试剂盒能够从高度加工的油中分离出可扩增的基因组DNA。针对向日葵特异性 基因开发了新型单重、双重和巢式PCR系统，用于高效鉴定向日葵。通过单重PCR揭示了新的向日葵DNA标记。改良CTAB和巢式PCR的组合被证明是一种可靠、快速且经济高效的技术，可用于检测700微升高度加工油（包括精炼油和使用过的烹饪油）中的微量向日葵。该研究将有助于食品工业和能源部门，因为所开发的方法可用于食品和生物柴油生产中的油真实性检测。
