使用RNA Later进行荧光激活细胞分选（FACS），以尽量减少RNA降解以及参与初始宿主-微生物相互作用的细胞中mRNA表达的扰动。
Fluorescence activated cell sorting (FACS) using RNAlater to minimize RNA degradation and perturbation of mRNA expression from cells involved in initial host microbe interactions.
作者信息
Nishimoto Kevin P, Newkirk Daniel, Hou Stephen, Fruehauf John, Nelson Edward L
机构信息
Molecular Biology and Biochemistry, School of Biological Sciences, University of California, Irvine, Irvine, CA 92697, USA.
出版信息
J Microbiol Methods. 2007 Jul;70(1):205-8. doi: 10.1016/j.mimet.2007.03.022. Epub 2007 Apr 12.
Host microbe interactions frequently involve specific cellular tropism. Accurate characterization of cells involved in these initial interactions is complicated by the response to the microbe. We describe a method utilizing RNAlater for Fluorescence Activated Cell Sorting of these critical cells that minimizes the downstream perturbation in the gene expression profile.
宿主与微生物的相互作用通常涉及特定的细胞嗜性。由于微生物的反应，准确表征参与这些初始相互作用的细胞变得复杂。我们描述了一种利用RNA Later对这些关键细胞进行荧光激活细胞分选的方法，该方法可将基因表达谱中的下游干扰降至最低。
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