作为芘激发复合物支架的RNA

RNA as scaffold for pyrene excited complexes.

作者信息

Grünewald Christian, Kwon Taewoo, Piton Nelly, Förster Ute, Wachtveitl Josef, Engels Joachim W

机构信息

Institute of Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 7, 60348 Frankfurt, Germany.

出版信息

Bioorg Med Chem. 2008 Jan 1;16(1):19-26. doi: 10.1016/j.bmc.2007.04.058. Epub 2007 May 3.

DOI:10.1016/j.bmc.2007.04.058

PMID:17512739

Abstract

Synthesis and spectral properties of 1-ethynylpyrene base modified RNA are reported. The fluorophore attached to the 2-position of adenosine is directed into the easily accessible minor groove in RNA. Through an intermolecular interaction of the pyrene residues in twofold labelled RNA, single and double strands can be distinguished by their fluorescence maxima around 450 and 480 nm, respectively. This behaviour allows the kinetic investigation of RNA hybridisation and folding by fluorescence spectroscopy.

摘要

报道了1-乙炔基芘碱基修饰的RNA的合成及其光谱性质。连接在腺苷2位的荧光团指向RNA中易于接近的小沟。通过双链标记RNA中芘残基的分子间相互作用，单链和双链RNA可分别通过其在450nm和480nm左右的荧光最大值来区分。这种特性使得通过荧光光谱法对RNA杂交和折叠进行动力学研究成为可能。