Wang Li, Zhang Zhujun
Department of Chemistry, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.
Anal Chim Acta. 2007 Jun 5;592(2):115-20. doi: 10.1016/j.aca.2007.04.007. Epub 2007 Apr 8.
Sensitive, rapid and inexpensive chemiluminescence (CL) imaging has been developed based on molecular imprinted polymer (MIP) sensing elements. Imprinted uniform microspheres were synthesized by precipitation polymerization. Microtiter plates (96 wells) were coated with polymer microspheres imprinted with trans-resveratrol, which were fixed in place using poly(vinyl alcohol) (PVA) as glue. The amount of polymer-bound trans-resveratrol was quantified using imidazole (IMZ)-catalyzed peroxyoxalate chemiluminescence (PO-CL) reaction. The light produced was then measured with a high-resolution CCD camera. Calibration curve corresponding to analyte concentration ranging from 0.3 to 25 microg mL(-1) was obtained with a limit of detection 0.1 microg mL(-1). These results showed that the MIP-based CL imaging sensor can become a useful analytical tool for quick simultaneous detection of trans-resveratrol in a large number of real samples.
