School of Chemical and Biological Science and Engineering, Yantai University, People's Republic of China.
Luminescence. 2012 Jul-Aug;27(4):297-301. doi: 10.1002/bio.1350. Epub 2011 Oct 12.
A novel method was developed using molecular imprinting technology (MIT) coupled with flow-injection chemiluminescence (FI-CL) for highly sensitive detection of phenformin hydrochloride (PH). The phenformin imprinted polymer was synthesized with methacrylic acid (MAA) as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. Newly synthesized molecularly imprinted polymer (MIP) particles were packed into a column as a selective recognition element for determination of PH. A CL method for the determination of PH was developed based on the CL reaction of PH with N-bromosuccinimide sensitized by eosin Y in basic media. The optimization of detection conditions was investigated. The CL intensity responded linearly to the concentration of PH in the range 0.09-2.0 µg/mL, with a correlation coefficient of 0.9920. The detection limit was 0.031 µg/mL. The relative standard deviation for the determination of 1.0 µg/mL PH solution was 1.0% (n = 11). The method was applied to the determination of PH in urine samples, with satisfactory results.
一种新的方法是使用分子印迹技术（MIT）与流动注射化学发光（FI-CL）相结合，用于盐酸苯乙双胍（PH）的高灵敏度检测。采用甲基丙烯酸（MAA）作为功能单体和乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂合成了苯乙双胍印迹聚合物。将新合成的分子印迹聚合物（MIP）颗粒填充到柱中作为选择性识别元件，用于 PH 的测定。基于 PH 在碱性介质中与吖啶黄敏化的 N-溴代丁二酰亚胺的 CL 反应，建立了测定 PH 的 CL 方法。考察了检测条件的优化。CL 强度与 PH 的浓度在 0.09-2.0 µg/mL 范围内呈线性响应，相关系数为 0.9920。检测限为 0.031 µg/mL。测定 1.0 µg/mL PH 溶液的相对标准偏差为 1.0%（n = 11）。该方法应用于尿样中 PH 的测定，结果令人满意。
