School of Chemistry and Chemical Engineering, University of Jinan, No. 106 Jiwei Road, Jinan 250022, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Feb;86:456-60. doi: 10.1016/j.saa.2011.10.068. Epub 2011 Nov 4.
A novel molecular imprinting-chemiluminescence (MIP-CL) sensor for the determination of L-phenylalanine (Phe) using molecularly imprinted polymer (MIP) as recognition element is reported. The Phe-MIP was synthesized using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile (AIBN) as initiator and the polymers' properties were characterized. Then the synthesized MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of Phe in the range 1.3 × 10(-6) to 5.44 × 10(-4) mol/L with a detection limit of 6.23 × 10(-7) mol/L (3σ), which is lower than that of conventional methods. The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of Phe in samples.
一种基于分子印迹-化学发光（MIP-CL）原理的新型传感器，用于测定 L-苯丙氨酸（Phe）。该传感器以分子印迹聚合物（MIP）作为识别元件，以丙烯酰胺（AM）作为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂，2,2-偶氮二异丁腈（AIBN）作为引发剂，合成了 Phe-MIP。然后，将合成的 MIP 作为识别元件，装入流动池，建立了一种新型的流动注射 CL 传感器。CL 强度与 Phe 的浓度在 1.3×10(-6)至 5.44×10(-4) mol/L 范围内呈线性响应，检测限为 6.23×10(-7) mol/L（3σ），低于常规方法。该传感器可重复使用，对 CL 分析具有较高的灵敏度和选择性。因此，该新型 MIP-CL 传感器已成功应用于样品中 Phe 的测定。
