College of Chemistry and Materials Science, Shaanxi Normal University, Xi'an, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Jan;75(1):77-82. doi: 10.1016/j.saa.2009.09.044. Epub 2009 Oct 6.
A novel flow-injection chemiluminescence (CL) method for the determination of dihydralazine sulfate (DHZS) is described. The method is based on the reaction of luminol and diperiodatocuprate (K(2)[Cu(H(2)IO(6))(OH)(2)], DPC) in alkaline medium to emit CL, which is greatly enhanced by DHZS. The possible CL mechanism was first proposed based on the kinetic characteristic, CL spectrum and UV spectra. The optimum condition for the CL reaction was in detail studied using flow-injection system. The experiments indicated that under optimum condition, the CL intensity was linearly related to the concentration of DHZS in the range of 7.0x10(-9) to 8.6x10(-7) g mL(-1) with a detection limit (3sigma) of 2.1x10(-9) g mL(-1). The proposed method had good reproducibility with the relative standard deviation 3.1% (n=7) for 5.2x10(-8) g mL(-1) of DHZS. This method has the advantages of simple operation, fast response and high sensitivity. The special advantage of the system is that very low concentration of luminol can react with DPC catalyzed by DHZS to get excellent experiment results. And CL cannot be observed nearly when luminol with same concentration reacts with other oxidants, so luminol-DPC system has higher selectivity than other luminol CL systems. The method has been successfully applied to determine DHZS in serum.
一种新的流动注射化学发光（CL）法用于测定硫酸二肼屈嗪（DHZS）。该方法基于在碱性介质中鲁米诺与过二碘酸钠铜（K（2）[Cu（H（2）IO（6））（OH）（2）]，DPC）的反应产生 CL，DHZS 大大增强了 CL 的发光强度。首先根据动力学特性、CL 光谱和 UV 光谱提出了可能的 CL 机制。使用流动注射系统详细研究了 CL 反应的最佳条件。实验表明，在最佳条件下，CL 强度与 DHZS 的浓度在 7.0x10（-9）至 8.6x10（-7）g mL（-1）范围内呈线性关系，检测限（3sigma）为 2.1x10（-9）g mL（-1）。该方法具有良好的重现性，对于 5.2x10（-8）g mL（-1）DHZS 的相对标准偏差为 3.1%（n=7）。该方法具有操作简单、响应快、灵敏度高的优点。该系统的一个特殊优点是，非常低浓度的鲁米诺可以与 DHZS 催化的 DPC 反应，得到极好的实验结果。并且当相同浓度的鲁米诺与其他氧化剂反应时，几乎观察不到 CL，因此鲁米诺-DPC 体系比其他鲁米诺 CL 体系具有更高的选择性。该方法已成功应用于血清中 DHZS 的测定。
