Buchalla W, Lennon A M, Becker K, Lucke T, Attin T
Department for Preventive Dentistry, Periodontology and Cariology, Center for Dentistry, Oral Medicine and Cranio-Maxillofacial Surgery, University of Zürich, Switzerland.
Arch Oral Biol. 2007 Oct;52(10):932-7. doi: 10.1016/j.archoralbio.2007.04.002. Epub 2007 May 21.
Polishing generates a smear layer (SL) on in vitro dentin samples that may influence fluoride uptake. We tested two hypotheses: SL increases fluoride uptake in superficial dentin (H1) and decreases fluoride uptake in deeper layers (H2) irrespectively of the amount of fluoride administered.
Polished bovine dentin with SL present and removed by four methods (5% tannic acid, 20s [TA]; 17% EDTA, 120 s; 38% phosphoric acid, 60s [PA]; and 10s air polishing) was fluoridated with 1200 or 12000 ppm F (NaF) solution (pH 4.0).
Scanning electron microscopy (SEM) revealed that aggressiveness of SL removal varied by method from leaving SL patches behind (TA) to collagen exposure (PA). SL increased KOH-soluble and structurally bound fluoride uptake into superficial and deeper layers compared to SL free surfaces (except PA) following 1200 ppm, but not 12000 ppm fluoridation.
Presence of SL and surface conditions influence dentin fluoride uptake depending on fluoride concentration administered.
在体外牙本质样本上进行抛光会产生玷污层（SL），这可能会影响氟摄取。我们测试了两个假设：无论给予的氟含量如何，SL都会增加表层牙本质中的氟摄取（假设1），并减少深层牙本质中的氟摄取（假设2）。
通过四种方法（5% 鞣酸，20秒 [TA]；17% 乙二胺四乙酸，120秒；38% 磷酸，60秒 [PA]；以及10秒空气抛光）去除或保留玷污层的抛光牛牙本质，用1200或12000 ppm F（氟化钠）溶液（pH 4.0）进行氟化处理。
扫描电子显微镜（SEM）显示，去除玷污层的侵蚀性因方法而异，从留下玷污层斑块（TA）到暴露胶原蛋白（PA）。与无玷污层的表面相比，在1200 ppm氟化处理后，但不是12000 ppm氟化处理后，玷污层增加了表层和深层中氢氧化钾可溶性氟和结构结合氟的摄取（PA除外）。
玷污层的存在和表面状况会根据所给予的氟浓度影响牙本质氟摄取。
