用于金黄色葡萄球菌的改良单拷贝整合载体。
Improved single-copy integration vectors for Staphylococcus aureus.
作者信息
Luong Thanh T, Lee Chia Y
机构信息
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham St. Mail Slot 511, Little Rock, AR 72205, United States.
出版信息
J Microbiol Methods. 2007 Jul;70(1):186-90. doi: 10.1016/j.mimet.2007.04.007. Epub 2007 Apr 24.
Abstract
We have previously reported the construction of Staphylococcus aureus single-copy integration vectors based on the lysogenic bacteriophage L54a site-specific recombination system. These vectors can replicate autonomously in Escherichia coli, which allows DNA manipulations. In S. aureus, the vectors, which do not possess staphylococcal replication function, can only be maintained by integrating into the chromosome. However, the original vectors have limited cloning sites and do not have protection from potential transcription of external promoters. Here we report the improved version of these vectors that circumvent these shortcomings. In addition, a second integration site based on the bacteriophage phi11 site-specific recombination system has been added such that the vectors can integrate either at the L54a attachment site or at the phi11 attachment site.
摘要
我们之前报道过基于溶原性噬菌体L54a位点特异性重组系统构建金黄色葡萄球菌单拷贝整合载体。这些载体可在大肠杆菌中自主复制，便于进行DNA操作。在金黄色葡萄球菌中，这些不具备葡萄球菌复制功能的载体只能通过整合到染色体中得以维持。然而，原始载体的克隆位点有限，且无法抵御外部启动子的潜在转录。在此，我们报道了改进后的载体版本，克服了这些缺点。此外，还添加了基于噬菌体phi11位点特异性重组系统的第二个整合位点，使得载体能够整合到L54a附着位点或phi11附着位点。
相似文献
1
Improved single-copy integration vectors for Staphylococcus aureus.用于金黄色葡萄球菌的改良单拷贝整合载体。
J Microbiol Methods. 2007 Jul;70(1):186-90. doi: 10.1016/j.mimet.2007.04.007. Epub 2007 Apr 24.
2
Extent of the DNA sequence required in integration of staphylococcal bacteriophage L54a.葡萄球菌噬菌体L54a整合所需的DNA序列长度
J Bacteriol. 1989 Mar;171(3):1652-7. doi: 10.1128/jb.171.3.1652-1657.1989.
3
Construction of single-copy integration vectors for Staphylococcus aureus.金黄色葡萄球菌单拷贝整合载体的构建
Gene. 1991 Jul 15;103(1):101-5. doi: 10.1016/0378-1119(91)90399-v.
4
Single-copy vectors for integration at the SaPI1 attachment site for Staphylococcus aureus.用于整合到金黄色葡萄球菌SaPI1附着位点的单拷贝载体。
Plasmid. 2014 Nov;76:1-7. doi: 10.1016/j.plasmid.2014.08.001. Epub 2014 Sep 2.
5
New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli.基于新型穿梭载体的表达系统，用于在金黄色葡萄球菌和大肠杆菌中生成多组氨酸标签融合蛋白。
Appl Environ Microbiol. 2015 May 1;81(9):3243-54. doi: 10.1128/AEM.03803-14. Epub 2015 Mar 6.
6
A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome.一种单拷贝整合载体，可整合到金黄色葡萄球菌染色体上的一个工程位点。
BMC Res Notes. 2012 Jan 5;5:5. doi: 10.1186/1756-0500-5-5.
7
Localization of prophages of serological group B and F on restriction fragments defined in the restriction map of Staphylococcus aureus NCTC 8325.血清学B组和F组原噬菌体在金黄色葡萄球菌NCTC 8325限制酶切图谱所定义的限制片段上的定位。
FEMS Microbiol Lett. 1996 Oct 1;143(2-3):203-10. doi: 10.1111/j.1574-6968.1996.tb08481.x.
8
Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites.葡萄球菌噬菌体L54a通过位点特异性重组实现整合：附着位点的结构分析
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5474-8. doi: 10.1073/pnas.83.15.5474.
9
Novel organization of the site-specific integration and excision recombination functions of the Staphylococcus aureus serotype F virulence-converting phages phi 13 and phi 42.金黄色葡萄球菌F血清型毒力转换噬菌体phi 13和phi 42位点特异性整合与切除重组功能的新组织形式
Mol Microbiol. 1995 Jun;16(5):877-93. doi: 10.1111/j.1365-2958.1995.tb02315.x.
10
Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.葡萄球菌噬菌体L54a整合酶基因和切除酶基因的核苷酸序列及遗传特征
J Bacteriol. 1989 Aug;171(8):4146-53. doi: 10.1128/jb.171.8.4146-4153.1989.
引用本文的文献
1
Global changes in virulence and metabolism during colonization of healthy skin.健康皮肤定植过程中致病性和代谢的整体变化。
Infect Immun. 2025 Apr 8;93(4):e0002825. doi: 10.1128/iai.00028-25. Epub 2025 Mar 21.
2
The Staphylococcus aureus non-coding RNA IsrR regulates TCA cycle activity and virulence.金黄色葡萄球菌非编码RNA IsrR调节三羧酸循环活性和毒力。
Nucleic Acids Res. 2025 Feb 8;53(4). doi: 10.1093/nar/gkae1243.
3
PcdA promotes orthogonal division plane selection in Staphylococcus aureus.PcdA 促进金黄色葡萄球菌的正交分裂平面选择。
Nat Microbiol. 2024 Nov;9(11):2997-3012. doi: 10.1038/s41564-024-01821-8. Epub 2024 Oct 28.
4
The small non-coding RNA IsrR regulates TCA cycle activity and virulence.小非编码RNA IsrR调节三羧酸循环活性和毒力。
bioRxiv. 2024 Jul 4:2024.07.03.601953. doi: 10.1101/2024.07.03.601953.
5
Host-derived protease promotes aggregation of by cleaving the surface protein SasG.宿主来源的蛋白酶通过切割表面蛋白 SasG 促进 的聚集。
mBio. 2024 Apr 10;15(4):e0348323. doi: 10.1128/mbio.03483-23. Epub 2024 Mar 21.
6
S. aureus drives itch and scratch-induced skin damage through a V8 protease-PAR1 axis.金黄色葡萄球菌通过 V8 蛋白酶-PAR1 轴驱动瘙痒和搔抓诱导的皮肤损伤。
Cell. 2023 Nov 22;186(24):5375-5393.e25. doi: 10.1016/j.cell.2023.10.019.
7
Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation.多功能等位基因替换和自我切除整合载体，用于质粒基因组突变和互补。
Microbiol Spectr. 2024 Jan 11;12(1):e0338723. doi: 10.1128/spectrum.03387-23. Epub 2023 Nov 22.
8
Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus.铜离子抑制金黄色葡萄球菌的戊糖磷酸途径功能。
PLoS Pathog. 2023 May 26;19(5):e1011393. doi: 10.1371/journal.ppat.1011393. eCollection 2023 May.
9
Localized modulation of DNA supercoiling, triggered by the Shigella anti-silencer VirB, is sufficient to relieve H-NS-mediated silencing.由痢疾杆菌反沉默因子 VirB 引发的 DNA 超螺旋的局部调节足以缓解 H-NS 介导的沉默。
Nucleic Acids Res. 2023 May 8;51(8):3679-3695. doi: 10.1093/nar/gkad088.
10
Unravelling the physiological roles of mazEF toxin-antitoxin system on clinical MRSA strain by CRISPR RNA-guided cytidine deaminase.通过 CRISPR RNA 引导的胞嘧啶脱氨酶揭示临床耐甲氧西林金黄色葡萄球菌菌株中 mazEF 毒素-抗毒素系统的生理作用。
J Biomed Sci. 2022 May 7;29(1):28. doi: 10.1186/s12929-022-00810-5.
本文引用的文献
1
The arl locus positively regulates Staphylococcus aureus type 5 capsule via an mgrA-dependent pathway.arl基因座通过一种依赖mgrA的途径正向调控金黄色葡萄球菌5型荚膜。
Microbiology (Reading). 2006 Oct;152(Pt 10):3123-3131. doi: 10.1099/mic.0.29177-0.
2
Novel cassette-based shuttle vector system for gram-positive bacteria.用于革兰氏阳性菌的新型基于盒式的穿梭载体系统。
Appl Environ Microbiol. 2004 Oct;70(10):6076-85. doi: 10.1128/AEM.70.10.6076-6085.2004.
3
Comparative analysis of the genomes of the temperate bacteriophages phi 11, phi 12 and phi 13 of Staphylococcus aureus 8325.金黄色葡萄球菌8325的温和噬菌体phi 11、phi 12和phi 13基因组的比较分析
Gene. 2002 May 1;289(1-2):109-18. doi: 10.1016/s0378-1119(02)00481-x.
4
Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains.用于铜绿假单胞菌的整合能力强的质粒：位点特异性整合及其在报告菌株和表达菌株工程中的应用
Plasmid. 2000 Jan;43(1):59-72. doi: 10.1006/plas.1999.1441.
5
Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.利用大肠杆菌乳糖阻遏物和操纵基因控制枯草芽孢杆菌中的基因表达。
Proc Natl Acad Sci U S A. 1984 Jan;81(2):439-43. doi: 10.1073/pnas.81.2.439.
6
The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage.毒性休克综合征外毒素结构基因不能被前噬菌体检测到地传播。
Nature. 1983;305(5936):709-12. doi: 10.1038/305709a0.
7
Improved single and multicopy lac-based cloning vectors for protein and operon fusions.用于蛋白质和操纵子融合的改进型基于乳糖操纵子的单拷贝和多拷贝克隆载体。
Gene. 1987;53(1):85-96. doi: 10.1016/0378-1119(87)90095-3.
8
Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene.葡萄球菌脂肪酶的溶原性转变是由噬菌体L54a基因组插入脂肪酶结构基因引起的。
J Bacteriol. 1986 May;166(2):385-91. doi: 10.1128/jb.166.2.385-391.1986.
9
Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites.葡萄球菌噬菌体L54a通过位点特异性重组实现整合：附着位点的结构分析
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5474-8. doi: 10.1073/pnas.83.15.5474.
10
Construction of single-copy integration vectors for Staphylococcus aureus.金黄色葡萄球菌单拷贝整合载体的构建
Gene. 1991 Jul 15;103(1):101-5. doi: 10.1016/0378-1119(91)90399-v.
Zotero 插件