Sukdeo Nicole, Honek John F
Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada N2L 3G1.
Biochim Biophys Acta. 2007 Jun;1774(6):756-63. doi: 10.1016/j.bbapap.2007.04.005. Epub 2007 Apr 20.
The glyoxalase (Glx) system is a critical detoxification enzyme system that is widely distributed in prokaryotic and eukaryotic organisms. Glyoxalase I (GlxI), the first enzyme in the system, is a divalent metal-ion dependent lyase (isomerizing), and its homologs have recently been categorized into two metal activation classes which are either Zn2+-dependent or non-Zn2+ dependent (Ni2+-/Co2+-activated). The latter class encompasses enzymes of predominantly bacterial origin. We have identified two genes in Pseudomonas aeruginosa PAO1 encoding glyoxalase I enzymes in addition to the gloA1 sequence recently reported and characterized. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene remarkably encodes a Zn2+-dependent homolog. To our knowledge this is the first report of a eubacterial species with several GlxI encoding genes, and also of an organism possessing GlxI enzymes from both metal activation classes.
乙二醛酶（Glx）系统是一种关键的解毒酶系统，广泛分布于原核生物和真核生物中。乙二醛酶I（GlxI）是该系统中的第一种酶，是一种二价金属离子依赖性裂合酶（异构化），其同源物最近被分为两种金属激活类别，即锌离子依赖性或非锌离子依赖性（镍离子/钴离子激活）。后一类主要包括细菌来源的酶。除了最近报道和表征的gloA1序列外，我们还在铜绿假单胞菌PAO1中鉴定出两个编码乙二醛酶I的基因。gloA1和gloA2基因编码非锌离子依赖性乙二醛酶I，而gloA3基因显著编码一种锌离子依赖性同源物。据我们所知，这是关于具有多个编码乙二醛酶I基因的真细菌物种以及拥有来自两种金属激活类别的乙二醛酶I的生物体的首次报道。
