Rooney W D, Springer C S
Department of Chemistry, State University, Stony Brook, NY 11794-3400.
NMR Biomed. 1991 Oct;4(5):209-26. doi: 10.1002/nbm.1940040502.
An extensive protocol for the study of tissue resonances of spin 3/2 nuclei is described. The roles of the most relevant multiple pulse experiments are indicated. Their theory is organized in terms of irreducible tensor operators and the pulse and quadrupolar relaxation transfer functions which relate them for a type c spectrum. A systematic approach to the interpretation of the temperature and/or magnetic field dependences of all six of the relaxation rate constants of the resonance of a single population of isolated spins in fast exchange, and giving rise to a type c spectrum, is presented. An experimental calibration and an application of this protocol are presented in an accompanying paper. The comprehensive method we describe has a number of practical benefits in the interpretation of the physiological spectra obtained from conventional one pulse experiments. A consideration of the appropriate transverse relaxation transfer function leads to an analytical expression for the heretofore empirical NMR visibility factor. This includes factors which account for relaxation during the receiver 'dead' time and relaxation during the pulse itself. Also, consideration of realistic transverse relaxation times likely to be observed in tissue leads to a reasonable strategy for the quantitative resolution and integration of in vivo spectra obtained in the presence of hyperfine shift reagents.
本文描述了一种用于研究自旋3/2核组织共振的广泛方案。指出了最相关的多脉冲实验的作用。其理论是根据不可约张量算符以及与c型谱相关的脉冲和四极弛豫传递函数来组织的。本文提出了一种系统的方法，用于解释在快速交换中孤立自旋单一群的共振的所有六个弛豫速率常数的温度和/或磁场依赖性，并产生c型谱。随附论文中介绍了该方案的实验校准和应用。我们所描述的综合方法在解释从传统单脉冲实验获得的生理光谱方面具有许多实际益处。对适当的横向弛豫传递函数的考虑导致了迄今为止经验性的NMR可见度因子的解析表达式。这包括在接收器“死”时间内的弛豫和脉冲本身期间的弛豫的因素。此外，考虑在组织中可能观察到的实际横向弛豫时间，会得出在存在超精细位移试剂的情况下获得的体内光谱的定量分辨率和积分的合理策略。
