乳酸乳球菌稳定期的荧光素酶检测
Luciferase detection during stationary phase in Lactococcus lactis.
作者信息
Bachmann Herwig, Santos Filipe, Kleerebezem Michiel, van Hylckama Vlieg Johan E T
机构信息
NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, 6710 BA Ede, The Netherlands.
出版信息
Appl Environ Microbiol. 2007 Jul;73(14):4704-6. doi: 10.1128/AEM.02807-06. Epub 2007 May 18.
Abstract
The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.
摘要
由luxAB编码的细菌荧光素酶的发光信号强烈依赖于宿主细胞的代谢状态，这限制了该报告系统仅用于代谢活跃的细菌。在此我们表明，在乳酸乳球菌的稳定期细胞中，通过向全细胞检测系统中添加核黄素或黄素单核苷酸，荧光素酶的检测得到显著改善。
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