Wolff C M, Stiegler P, Baltzinger M, Meyer D, Ghysdael J, Stéhelin D, Befort N, Remy P
Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Cell Growth Differ. 1991 Sep;2(9):447-56.
In a general approach to identify genes important in the control of genetic expression during development of Xenopus laevis, two complementary DNAs corresponding to two different c-ets-2 genes were cloned and sequenced. One of these complementary DNAs appears to be almost full length. The two variant genes differ in their overlapping sequences by 87 nucleotide substitutions, leading to 17 amino acid modifications in the proteins, 8 of them being conservative. All but one of these changes map outside of the 142 COOH-terminal residues, a region critical for nuclear localization and DNA binding in the ets proteins. Features potentially important for the biological activity of the gene products are conserved. Two transcripts (3.2 and 1.7 kilobases) with maternal characteristics are detected at a constant level from stages II/III of oogenesis to stage 10 of embryogenesis. They later decline to hardly detectable levels at stages 30-40. Variable amounts of the same transcripts are observed in many adult tissues. All of these characteristics support the idea that the ets-2 gene products play an important role during embryogenesis, as well as in adult life. Indeed, they act as ubiquitous transcriptional activators, as recently demonstrated by several investigators (C. V. Gunther, J. A. Nye, R. S. Bryner, and B. J. Graves, Genes & Dev., 4: 667-679, 1990; R. Bosselut, J.F. Duvall, A. Gegonne, M. Bailly, A. Hemar, J. Brady, and J. Ghysdael, EMBO J., 9: 3137-3144, 1990; R. Wasylyk, C. Wasylyk, P. Florès, A. Bègue, D. Leprince, and D. Stéhelin, Nature (Lond.), 346: 191-193, 1990).
在一种用于鉴定非洲爪蟾发育过程中对基因表达调控重要的基因的常规方法中，克隆并测序了对应于两个不同c-ets-2基因的两个互补DNA。其中一个互补DNA似乎几乎是全长的。这两个变异基因在其重叠序列中有87个核苷酸替换，导致蛋白质中有17个氨基酸修饰，其中8个是保守的。除了一个变化外，所有这些变化都位于142个COOH末端残基之外，该区域对ets蛋白的核定位和DNA结合至关重要。基因产物的生物学活性可能重要的特征是保守的。从卵子发生的II/III期到胚胎发生的第10阶段，以恒定水平检测到两种具有母体特征的转录本（3.2和1.7千碱基）。它们随后在30 - 40阶段下降到几乎不可检测的水平。在许多成年组织中观察到相同转录本的量各不相同。所有这些特征都支持ets-2基因产物在胚胎发生以及成年生活中起重要作用的观点。实际上，它们作为普遍存在的转录激活因子发挥作用，这一点最近已被几位研究者证实（C.V.冈瑟、J.A.奈伊、R.S.布赖纳和B.J.格雷夫斯，《基因与发育》，4: 667 - 679，1990；R.博塞卢特、J.F.杜瓦尔、A.热贡内、M.贝利、A.埃马尔、J.布雷迪和J.吉斯代尔，《欧洲分子生物学组织杂志》，9: 3137 - 3144，1990；R.瓦西利克、C.瓦西利克、P.弗洛雷斯、A.贝盖、D.勒普林斯和D.斯特赫林，《自然》（伦敦），346: 191 - 193，1990）。
