Chen Hsien-Tung, Yao Chun-Hsu, Chao Pei-Dawn Lee, Hou Yu-Chi, Chiang Hsiu-Mei, Hsieh Chang-Chi, Ke Cherng-Jyh, Chen Yueh-Sheng
Laboratory of Biomaterials, Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan.
J Biomed Mater Res B Appl Biomater. 2008 Jan;84(1):256-62. doi: 10.1002/jbm.b.30868.
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth factor (NGF)-mediated neurite outgrowth and the expression of synapsin I from PC12 cells. In the in vivo study, silicone rubber chambers filled with the SMP were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, animals from the groups treated with the SMP had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only. These results suggest that the serum metabolites of Pueraria lobata can be a potential nerve growth-promoting factor.
本研究对葛根大鼠血清代谢产物（SMP）促进周围神经再生进行了体外和体内评估。在体外研究中，我们发现SMP显著增强了神经生长因子（NGF）介导的PC12细胞神经突生长和突触素I的表达。在体内研究中，用填充有SMP的硅橡胶室桥接大鼠10毫米坐骨神经缺损。8周结束时，与仅接受生理盐水的对照组相比，接受SMP治疗组的动物神经结构相对更成熟，有髓轴突数量、神经内膜面积和总神经面积的平均值更大。这些结果表明，葛根血清代谢产物可能是一种潜在的神经生长促进因子。
