Lu Ming-Chin, Yao Chun-Hsu, Wang Ssu-Hung, Lai Yen-Liang, Tsai Chin-Chuan, Chen Yueh-Sheng
School of Post Baccalaureate Chinese Medicine, Changhua Christian Hospital, Changhua.
J Trauma. 2010 Feb;68(2):434-40. doi: 10.1097/TA.0b013e31819adb38.
This study provides in vitro and in vivo evaluation of Astragalus membranaceus (AM) on peripheral nerve regeneration.
In the in vitro study, we analyzed the effects of AM on cell differentiation and neurite outgrowth by using a PC12 cell model. In the in vivo study, silicone rubber chambers filled with the AM water extract were used to bridge a 10-mm sciatic nerve defect in rats.
We found that the AM water extract caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth and the expression of growth-associated protein 43 from PC12 cells. Animals from the groups treated with the AM for 8 weeks had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only.
These results suggest that the silicone rubber tubes present a good framework for the nerve fibers to regenerate across the gap, and the AM extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of axons in the peripheral nerve.
本研究对黄芪在外周神经再生方面进行了体外和体内评估。
在体外研究中，我们使用PC12细胞模型分析了黄芪对细胞分化和神经突生长的影响。在体内研究中，将填充有黄芪水提取物的硅橡胶室用于桥接大鼠10毫米的坐骨神经缺损。
我们发现黄芪水提取物显著增强了神经生长因子介导的PC12细胞神经突生长以及生长相关蛋白43的表达。与仅接受生理盐水的对照组相比，接受黄芪治疗8周的组中的动物具有相对更成熟的结构，其有髓轴突数量、神经内膜面积和总神经面积的平均值更大。
这些结果表明，硅橡胶管为神经纤维跨越间隙再生提供了良好的框架，黄芪提取物可能是一种潜在的神经生长促进因子，有助于外周神经轴突的生长。
